Open Access. Powered by Scholars. Published by Universities.®
Other Biochemistry, Biophysics, and Structural Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Arterioles (1)
- Bioluminescence (1)
- Calcium-binding protein (1)
- Conformational change (1)
- Critical limb ischemia (1)
-
- Cysteine mutagenesis (1)
- EF-hand (1)
- Endothelial cell (1)
- Endothelial to mesenchymal transition (1)
- Equilibrative nucleoside transport (1)
- Firefly luciferase (1)
- Fluorescence (1)
- Hydrophobicity (1)
- MagA (1)
- Magnetic resonance imaging (1)
- Methanethiosulfonate (1)
- Microvasculature (1)
- Mitochondrial calcium uniporter (1)
- Mitochondrial calcium uniporter dominant negative beta subunit (1)
- NMR spectroscopy (1)
- Nkx2.5 (1)
- Oligomerization (1)
- Protein design (1)
- Reporter gene expression (1)
- Skeletal muscle (1)
- Sodium iodide symporter (1)
- Solution nuclear magnetic resonance spectroscopy (1)
- Stability (1)
- Structure (1)
- Structure-function (1)
Articles 1 - 5 of 5
Full-Text Articles in Other Biochemistry, Biophysics, and Structural Biology
Uncovering The Structural Basis For Mitochondrial Calcium Uniporter Dominant Negative Beta Subunit (Mcub) Function, Megan L. Noble
Uncovering The Structural Basis For Mitochondrial Calcium Uniporter Dominant Negative Beta Subunit (Mcub) Function, Megan L. Noble
Electronic Thesis and Dissertation Repository
Mitochondrial calcium (Ca2+) uptake is regulated by the mitochondrial Ca2+ uniporter (MCU), a tetrameric channel that is regulated by interactions with several accessory proteins, including MCU dominant negative beta subunit (MCUb). MCUb inhibits Ca2+ uptake by assembling into the MCU complex and is incapable of forming a functional Ca2+ channel. The MCU amino (N)-terminal domain plays an essential role in controlling MCU structure and function and contains cation binding sites that, when bound by Ca2+ and magnesium (Mg2+), cause decreased MCU assembly and reduced mitochondrial Ca2+ uptake. MCU and MCUb contain …
Microvascular Stenosis In Critical Limb Ischemia: Role Of Partial Endothelial To Mesenchymal Transition, Jacqueline M. Chevalier
Microvascular Stenosis In Critical Limb Ischemia: Role Of Partial Endothelial To Mesenchymal Transition, Jacqueline M. Chevalier
Electronic Thesis and Dissertation Repository
Critical limb ischemia (CLI) is a widespread and debilitating manifestation of atherosclerosis. Unfortunately, revascularization strategies are often precluded or unsuccessful, resulting in amputation. A major reason for treatment failure is likely co-existing abnormalities in the microvasculature. However, the specific microvascular defects present in end-stage PAD in humans remain unknown.
The purpose of this study was to delineate abnormalities in the microvascular wall in the critically ischemic skeletal muscle of patients with CLI.
To elucidate the microvascular landscape in CLI, we studied human tibialis anterior and gastrocnemius muscles harvested from below-knee amputations of 10 individuals with CLI. Control muscles are from …
Towards The Development Of A Maga Reporter Gene Expression Construct For Magnetic Resonance Imaging, Karina Quiaoit
Towards The Development Of A Maga Reporter Gene Expression Construct For Magnetic Resonance Imaging, Karina Quiaoit
Electronic Thesis and Dissertation Repository
Development of methods to monitor cellular activity with magnetic resonance imaging (MRI) provides a powerful tool for studying disease progression and monitoring therapy. This thesis investigates components necessary for development of reporter gene expression for MRI, to track Nkx2.5 transcription factor activity during tumour growth and, in the future, to calibrate MR contrast against a well-known optical reporter gene, like firefly luciferase. In human MCF-7 breast cancer cells, Nkx2.5 is induced by all-trans retinoic acid (tRA) and activates a minimal promoter of the rat sodium iodide symporter (rNISmin) gene. Here, we used firefly luciferase reporter gene expression to examine a …
Human Equilibrative Nucleoside Transporter Subtype 1: Structure-Function Analysis Using Cysteine Mutagenesis And Thiol Modifying Techniques, Jamie Park
Electronic Thesis and Dissertation Repository
Human equilibrative nucleoside transporter 1 is the main mediator of bi-directional nucleoside flux and is found ubiquitously. Inhibitor and substrate interactions with ENT1 are known to be affected by cysteine-modifying reagents. Our aim was to investigate the importance of cysteine residues in hENT1 function and identify which residues were sensitive to thiol modification for further application of cysteine scanning mutagenesis on extracellular loop 5. Transporter function was assessed by the binding of [3H]NBMPR and the cellular uptake of [3H]2-chloroadenosine. Treatment of hENT1 with the neutral sulfhydryl-modifier methyl methanethiosulfonate (MMTS) enhanced [3H]NBMPR binding but decreased …
Identification Of Regions Responsible For The Open Conformation Of S100a10 Using Chimaeric S100a11/S100a10 Proteins, Liliana Santamaria-Kisiel
Identification Of Regions Responsible For The Open Conformation Of S100a10 Using Chimaeric S100a11/S100a10 Proteins, Liliana Santamaria-Kisiel
Electronic Thesis and Dissertation Repository
S100A11 is a dimeric, EF-hand calcium-binding protein. Calcium binding to S100A11 results in a large conformational change that uncovers a broad hydrophobic surface used to interact with phospholipid-binding proteins (annexins A1 and A2), and facilitate membrane vesiculation events. In contrast to other S100 proteins, S100A10 is unable to bind calcium due to deletion and substitution of calcium-ligating residues. Despite this, calcium-free S100A10 assumes an “open” conformation that is very similar to S100A11 in its calcium-bound state (Ca2+-S100A11). To understand how S100A10 is able to adopt an open conformation in the absence of calcium, seven chimeric proteins were constructed where regions …