Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Keyword
-
- CK2 (1)
- Cancer (1)
- Cell (1)
- Cell envelope (1)
- Cell signaling (1)
-
- Ciliated neurons (1)
- DAF-19 (1)
- Daf-19 (1)
- Development (1)
- Direct analysis in real time (1)
- Domain (1)
- F52D2.2 gene (1)
- F57B10.9 gene (1)
- Fourier Analysis (1)
- Fourier transform ion cyclotron resonance (1)
- Growth (1)
- Inhibitor (1)
- Kinase (1)
- Limit of Detection (1)
- Magna cum laude (1)
- Malathion (1)
- Mass Spectrometry (1)
- Mass spectrometry (1)
- Membrane (1)
- Mutation (1)
- Mycobacterium (1)
- Neurodegeneration (1)
- Neuronal developnment (1)
- Non-ciliated neurons (1)
- Non-replicating (1)
- Publication
- Publication Type
Articles 1 - 4 of 4
Full-Text Articles in Molecular Biology
Jmh Dissertation 2016.Pdf, Jennifer Hayashi
Jmh Dissertation 2016.Pdf, Jennifer Hayashi
Jennifer Hayashi
Characterization Of The Catalytic Ck2 Subunits With Substitutions At Residues Involved In Inhibitor Binding, Paul Desormeaux
Characterization Of The Catalytic Ck2 Subunits With Substitutions At Residues Involved In Inhibitor Binding, Paul Desormeaux
Electronic Thesis and Dissertation Repository
CK2 is a constitutively active, ubiquitously expressed and pleiotropic serine/threonine protein kinase that is implicated in many cellular functions including tumorigenesis. CK2 has two catalytic subunits, CK2a and CK2a’, that carry out its function in the cell. Previous studies have indicated that inhibitor-refractory mutants have been effective in recovering residual CK2 activity, in the presence of inhibitors, when compared to wild type CK2. Based on these observations, inhibitor-refractory mutants were created for both CK2a and CK2a’ and tested with various concentrations with two CK2-specific inhibitors, CX-4945 and inhibitor VIII. The CK2a triple mutant (V66A/I174A/H160D) was tested in inducible U2OS Flp-In …
The Role Of Daf-19 In Non-Ciliated Neurons: How Is Neural Development Regulated By Different Daf-19 Isoforms?, Zabdiel Ek Vazquez
The Role Of Daf-19 In Non-Ciliated Neurons: How Is Neural Development Regulated By Different Daf-19 Isoforms?, Zabdiel Ek Vazquez
Lawrence University Honors Projects
A degenerative disease-like phenotype, specifically reduction in synaptic protein levels in adult worms, is correlated with loss-of-function of the only RFX transcription factor gene, daf-19, in C. elegans. This gene encodes four known transcription factor isoforms, two of which are correlated with particular functions. The DAF-19C isoform activates genes responsible for cilia development, while DAF-19M is needed for cilia specification in males. A comparison of the transcriptome of daf-19 null and isogenic wild type adult worms suggests both positive and negative regulation of gene expression is correlated with the presence of DAF-19 proteins. We have assessed DAF-19 regulation …
Direct Analysis In Real Time (Dart) Of An Organothiophosphate At Ultrahigh Resolution By Fourier Transform Ion Cyclotron Resonance Mass Spectrometry And Tandem Mass Spectrometry, Laszlo Prokai, Stanley M. Stevens Jr.
Direct Analysis In Real Time (Dart) Of An Organothiophosphate At Ultrahigh Resolution By Fourier Transform Ion Cyclotron Resonance Mass Spectrometry And Tandem Mass Spectrometry, Laszlo Prokai, Stanley M. Stevens Jr.
Molecular Biosciences Faculty Publications
Direct analysis in real time (DART) is a recently developed ambient ionization technique for mass spectrometry to enable rapid and sensitive analyses with little or no sample preparation. After swab-based field sampling, the organothiophosphate malathion was analyzed using DART-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry (MS) and tandem mass spectrometry (MS/MS). Mass resolution was documented to be over 800,000 in full-scan MS mode and over 1,000,000 for an MS/MS product ion produced by collision-induced dissociation of the protonated analyte. Mass measurement accuracy below 1 ppm was obtained for all DART-generated ions that belonged to the test compound in the …