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Full-Text Articles in Molecular Biology
Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman
Discovery And Characterization Of A New Group Of Is10 Insertion Sequences, Rachel Marie Kinzelman
Theses and Dissertations
Insertion sequences (ISs) are small mobile genetic elements that can have significant impact on the genotype and phenotype of a host organism. Previous work in this laboratory revealed an insertion sequence that disrupted the luxA gene in Vibrio harveyi strain BCB451, knocking out light production. Phylogenetic analysis of this insertion sequence, dubbed IS451, reveals that it is in the IS10 family, but represents a novel variant that is only 79% identical to other known IS10 sequences. Twelve copies of IS451 were isolated from a genomic library and sequenced, and were found to be essentially identical, but located in dispersed chromosomal …
Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte
Lateral Replacement Of The Lux Operon In A Vibrio Isolated From The Intestine Of A Coral Reef Fish, Melissa Lee Whyte
Theses and Dissertations
In a screening of bioluminescent bacteria isolated from the intestines of coral reef fish, two strains (designated D6 and M1) were identified that have a luxA gene sequence significantly different from those of other Vibrio species. Phylogenetic analysis of several housekeeping genes, as well as toxR, shows that D6 and M1 branch within a bioluminescent clade (designated the “D1 group,” isolated at the same time and place as D6 and M1) that is a close sister group to Vibrio harveyi. However, whereas the luxA genes of the D1 group are >98% identical to V. harveyi luxA, the luxA genes of …