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Full-Text Articles in Molecular Biology
Protective Effects Of Pigmentation In Pseudomonas Aeruginosa: Insights On Pyomelanin Production And Inhibition By Ntbc, Laura Marie Ketelboeter
Protective Effects Of Pigmentation In Pseudomonas Aeruginosa: Insights On Pyomelanin Production And Inhibition By Ntbc, Laura Marie Ketelboeter
Theses and Dissertations
Pyomelanin is an extracellular, reddish-brown pigment produced by several environmental and pathogenic bacteria and fungi. It is derived from the phenylalanine/tyrosine catabolism pathway and is produced when homogentisate (HGA) is secreted from the cell, auto-oxidized, and self-polymerized. Point mutations or chromosomal deletions in hmgA, which encodes homogentisate 1,2-dioxygenase, result in the accumulation of HGA and subsequent pyomelanin production. My work showed that the pyomelanogenic Pseudomonas aeruginosa clinical isolate PA1111 had a chromosomal deletion of hmgA, while a second pyomelanogenic clinical isolate, DKN343, had a loss of function mutation in HmgA. The 4-hydroxyphenylpyruvate dioxygenase (Hpd) inhibiting compound 2-(2-nitro-4-trifluoromethylbenzoyl)-1,3-cyclohexanedione (NTBC) has been …
The Effect Of Transformed Escherichia Coli On The Mouse Intestine Microbiome: The Microbial Metabolic Enhancement Hypothesis, Bryar P. Kader
The Effect Of Transformed Escherichia Coli On The Mouse Intestine Microbiome: The Microbial Metabolic Enhancement Hypothesis, Bryar P. Kader
Senior Honors Theses
Metabolic disorders affect around thirty-four percent of the population in the United States. Among these disorders is lactose intolerance, which results from diminished production of the human lactase enzyme. This disorder and others like it are genetically determined and cannot be cured. However, the use of transformed bacteria implanted in the colon may provide a means by which the faulty pathway can be bypassed. To test whether transformed bacteria have the capability to aid in the digestion of normally indigestible compounds, a transformed strain of Escherichia coli overexpressing the beta-galactosidase enzyme encoded by the lacZ gene was colonized in the …