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Full-Text Articles in Molecular Biology
Efficiency Of The Targetron Gene Knockout System As A Transformative Protocol For The Mutagenesis Of Listeria Monocytogenes, Hilaree Noele Frazier
Efficiency Of The Targetron Gene Knockout System As A Transformative Protocol For The Mutagenesis Of Listeria Monocytogenes, Hilaree Noele Frazier
Online Theses and Dissertations
The process of using electroporation to introduce plasmid DNA into host cells is a valuable molecular technique that is increasingly employed in labs worldwide. Electroporators are generally small and relatively inexpensive, making them attractive systems to use for a variety of purposes. Electroporation protocols are numerous in the published literature and encompass all cell types, from prokaryotic bacterial cells to eukaryotic human cells. The TargeTron Gene Knockout System by Sigma-Aldrich is an affordable option for the electroporation of numerous bacterial species. However, its use in Listeria monocytogenes has not been extensively characterized. Here we sought to discuss the effectiveness of …