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The Drosophila Gypsy Insulator Supports Transvection In The Presence Of The Vestigial Enhancer, Todd Schoborg, Srilalitha Kuruganti, Ryan Rickels, Mariano Labrador

Faculty Publications and Other Works -- Biochemistry, Cellular and Molecular Biology

Though operationally defined as cis-regulatory elements, enhancers can also communicate with promoters on a separate homolog in trans, a mechanism that has been suggested to account for the ability of certain alleles of the same gene to complement one another in a process otherwise known as transvection. This homolog-pairing dependent process is facilitated in Drosophila by chromatin-associated pairing proteins, many of which remain unknown and their mechanism of action uncharacterized. Here we have tested the role of the gypsy chromatin insulator in facilitating pairing and communication between enhancers and promoters in trans using a transgenic eGFP reporter system …