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Molecular Biology Commons

Open Access. Powered by Scholars. Published by Universities.®

1990

Iowa State University

Plant Sciences

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

A Restriction Fragment Length Polymorphism Map Of Cochliobolus Heterostrophus , Tzy-Hwa Tzeng Jan 1990

A Restriction Fragment Length Polymorphism Map Of Cochliobolus Heterostrophus , Tzy-Hwa Tzeng

Retrospective Theses and Dissertations

An RFLP map was constructed for Cochliobolus heterostrophus, the causal agent of southern corn leaf blight, to facilitate the cloning of pathogenicity genes. A total of 128 RFLP and 4 phenotypic markers were analyzed. Of these, 126 were linked to other markers and delimited 944 cM, or, at a maximum, 58% of the total map length. Several RFLPs were found tightly linked to Tox1, a pathogenicity gene. A previously hypothesized translocation tightly linked to Tox1 was also detected;The numbers and sizes of the chromosomes of the two strains crossed to make the RFLP map were investigated using pulsed field ...


Cell-Wall Components And Lignin Biosynthesis In Forages , James Enderby Bidlack Jan 1990

Cell-Wall Components And Lignin Biosynthesis In Forages , James Enderby Bidlack

Retrospective Theses and Dissertations

Lignin is the major cell-wall (CW) component that lowers forage digestibility. Negative relationships between lignin and forage quality are caused by interactions and variability of lignin with other CW components. This study was conducted to better understand how lignin content, concentration, deposition, and change in concentration vary with other CW components in maturing stems and to provide preliminary information about phenylpropanoid metabolism in relation to lignification;Regrowth from basal stem tissue was sampled weekly or biweekly from greenhouse-grown forage grass and legume species. Samples were collected for a period of about 8 weeks and analyzed for fiber concentration and content ...