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Full-Text Articles in Molecular Biology
Epigenetic Characterization Of Human Retina Cells, Nicholas R. Dunham
Epigenetic Characterization Of Human Retina Cells, Nicholas R. Dunham
Senior Honors Projects, 2010-2019
DNA methylation is an epigenetic modifier that modulates gene expression in plant and vertebrate genomes. The aim of this study was to characterize the role of DNA methylation in the human retina, particularly within rod and cone photoreceptor retinal neurons. Previous studies investigating DNA methylation in murine retinal cells and retina-derived human retinoblastoma immortalized cell culture lines demonstrate an inverse relationship between DNA methylation and transcriptional activity. Here, we used gene-specific bisulfite pyrosequencing analysis to measure DNA methylation in the genomes of human ocular cells in an effort to characterize the role of this important epigenetic modifier. These results can …