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Molecular Biology Commons

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Theses/Dissertations

And developmental biology

Genetics and Genomics

1991

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley Jan 1991

Avena Phytochrome Mrna: In Vivo Localization, Degradation Products, And Half-Life , Kevin Andrew Seeley

Retrospective Theses and Dissertations

The purpose of this research is to determine the role that post-transcriptional processes play in the regulation of phytochrome (phy) steady-state messenger RNA (mRNA) levels. The control of etiolated phytochrome (phyA) and chlorophyll a/b binding protein (cab) gene transcription and steady-state mRNA levels are known to be regulated by the phyA signal transduction pathway. I performed a number of control experiments that indicate the degradation products detected in northern analysis of phyA mRNA were produced in vivo and were not an artifact of RNA isolation techniques. I have also shown that infrared (IR) irradiation has no appreciable effect on ...


Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton Jan 1991

Analysis Of Three Plasmids From Filamentous, Nitrogen-Fixing Cyanobacteria , Douglas Kevin Walton

Retrospective Theses and Dissertations

The cyanobacteria are an ancient and diverse group of prokaryotes capable of oxygenic photosynthesis. They display both unicellular and filamentous cell types and some have the ability to fix atmospheric dinitrogen, and the ability to differentiate specialized cells. In addition, many of the cyanobacteria possess plasmids. To date, only one plasmid, from a unicellular non-nitrogen-fixer, has been characterized in detail. The current lack of information concerning cyanobacterial plasmids, and their subsequent use as vectors, has hindered genetic analysis, particularly in the filamentous species;In this study, the properties of two plasmids and one plasmid origin of replication from filamentous, nitrogen-fixing ...