Open Access. Powered by Scholars. Published by Universities.®
- Discipline
-
- Biochemistry (6)
- Medicine and Health Sciences (4)
- Biochemical Phenomena, Metabolism, and Nutrition (2)
- Biology (2)
- Cell and Developmental Biology (2)
-
- Diseases (2)
- Fungi (2)
- Genetics and Genomics (2)
- Laboratory and Basic Science Research (2)
- Medical Sciences (2)
- Nutritional and Metabolic Diseases (2)
- Organisms (2)
- Biophysics (1)
- Cancer Biology (1)
- Cell Biology (1)
- Chemistry (1)
- Curriculum and Instruction (1)
- Dermatology (1)
- Education (1)
- Educational Assessment, Evaluation, and Research (1)
- Medical Specialties (1)
- Molecular Genetics (1)
- Molecular, Genetic, and Biochemical Nutrition (1)
- Nutrition (1)
- Oncology (1)
- Other Chemistry (1)
- Other Teacher Education and Professional Development (1)
- Keyword
-
- Biochemistry (2)
- Iron Regulation (2)
- Aft1 (1)
- Aft2 (1)
- Art (1)
-
- Bioinorganic catalysis (1)
- Bol2 (1)
- Cellular localization (1)
- Chlamydia protein associating with Death Domains (1)
- Chlamydia trachomatis (1)
- Communities in Harmony (1)
- Community Service (1)
- Compound I (1)
- Cytochrome P450 (1)
- Diiron (1)
- Drug development (1)
- Fungal Pathogen (1)
- Grx3 (1)
- Grx4 (1)
- Heme Substitution (1)
- High impact practice (1)
- Homeless (1)
- Integrative learning (1)
- MITF (1)
- Melanocyte Inducing Transcription Factor (1)
- Metabolic Disease (1)
- Molecular Biology (1)
- Music (1)
- Nitrosomonas europaea (1)
- Outcome (1)
Articles 1 - 8 of 8
Full-Text Articles in Molecular Biology
Purification And Functional Characterization Of The Iron-Responsive Transcription Factor Aft1 From C. Glabrata, Jade Ikahihifo-Bender
Purification And Functional Characterization Of The Iron-Responsive Transcription Factor Aft1 From C. Glabrata, Jade Ikahihifo-Bender
Senior Theses
Due to its unique ability to serve as both an electron donor and acceptor, iron is utilized as a co-factor for many biological processes, including electron transfer, oxygen binding, and vitamin synthesis. Iron is also a key factor during fungal infections as the human host and invading pathogens battle over limited iron pools. The primary iron-responsive transcription factor Aft1 in the opportunistic pathogenic yeast Candida glabrata responds to iron deficiency by activating expression of iron acquisition genes. However, the mechanisms for sensing intracellular iron levels and regulating Aft1 activity in response to iron are unknown. The C. glabrata iron regulation …
Lions, Tigers, And Hemes - Oh My! A Dynamic Look At The Electronic Effects Of Porphyrin Substitution On Cytochrome P450 Olet, Alexis J. Holwerda
Lions, Tigers, And Hemes - Oh My! A Dynamic Look At The Electronic Effects Of Porphyrin Substitution On Cytochrome P450 Olet, Alexis J. Holwerda
Senior Theses
OleT, a member of the CYP152 family of cytochrome P450s (CYPs), decarboxylates fatty acids using hydrogen peroxide as an oxidant. The resultant products are a terminal alkene and carbon dioxide. This C–C cleavage reaction is highly atypical for CYPs, which prototypically oxygenate substrates, and provides a potential means to enzymatically produce drop-in fuels. OleT contains a heme-iron cofactor that facilitates decarboxylation through the activation of hydrogen peroxide. The catalytic cycle, as determined by transient kinetics, includes two ferryl intermediates known as Compound I (Ole-I) and Compound II (Ole-II). Ole-I performs substrate hydrogen abstraction and subsequent single electron transfer to Ole-II …
Investigating The Redox Sensitivity Of Mitf Splice Variants, Rachel Berryman
Investigating The Redox Sensitivity Of Mitf Splice Variants, Rachel Berryman
Senior Theses
Within pigment-producing cells known as melanocytes, the transcription factor MITF is intimately involved in regulating genes associated with cell cycle maintenance and melanocyte differentiation. Research, however, has provided conflicting results on the relationship between the expression levels of MITF and melanocyte cell fate. To complicate matters, two splice variants of MITF exist, differing by only 18 base pairs. These variants have been observed at variable levels of expression in melanocyte and melanoma cells, raising the question as to their functional purpose. Building upon previous research by the Leachman/Cassidy lab that identified the redox sensitivity of MITF while additionally establishing a …
A Broadly Distributed, Novel Class Of Diiron Enzymes Involved In Para-Aminobenzoate Synthesis, Joseph M. Zambelas
A Broadly Distributed, Novel Class Of Diiron Enzymes Involved In Para-Aminobenzoate Synthesis, Joseph M. Zambelas
Senior Theses
The overall structure and ligand arrangement of diiron enzymes influence their function and catalytic potential, allowing for them to catalyze an expansive breadth of high energy transformations. The diiron enzyme CADD from Chlamydia trachomatis is involved in a novel biosynthetic pathway for p-aminobenzoic acid (pABA), a precursor for tetrahydrofolate. The unique ligand structure and reactivity of CADD and homolog NE1434 provide an intriguing means to contrast structure function relationships with other known diiron enzymes that activate dioxygen. In this work, studies of CADD and NE1434 orthologs and comparison to other diiron enzymes reveal several sequence motifs that are likely critical …
Cellular Localization Of Rad51d Mutant Proteins And The Application Of Art To Increase Scientific Literacy In America, Claire L. Chabot
Cellular Localization Of Rad51d Mutant Proteins And The Application Of Art To Increase Scientific Literacy In America, Claire L. Chabot
Senior Theses
Ovarian cancers are the leading cause of death from cancer of the female reproductive system. Approximately 50% of ovarian cancers have defects in the homologous recombination (HR) DNA repair pathway that is required for the repair of DNA double-stranded breaks. The status of HR genes, such as BRCA1, BRCA2, and the RAD51 family, contributes to ovarian cancer development as well as treatment decisions regarding chemotherapy, radiation, and immunotherapy. The overarching goal of this project is to identify new insights into HR that can integrate with Precision Medicine Initiatives and align with the goals of the Cancer Moonshot 2020 Program. I …
Characterizing Aft1/2-Grx3/4 Interaction And The Role Of Bol2 During Iron Regulation In Saccharomyces Cerevisiae, William Rivers
Characterizing Aft1/2-Grx3/4 Interaction And The Role Of Bol2 During Iron Regulation In Saccharomyces Cerevisiae, William Rivers
Senior Theses
Iron dysregulation has been linked to a variety of human diseases, such as anemia, Friedreich’s ataxia, X-linked sideroblastic anemia, sideroblastic-like microcytic anemia, and myopathy. Thus, it is vitally important to understand the mechanisms for regulating intracellular iron. Here, we use fluorescence microscopy techniques in live cells to study interactions of the yeast proteins Grx3/4, Aft1/2, and Bol2, which have been shown to be involved in turning off iron import when the cell has adequate iron. Modified versions of genes encoding these proteins have been incorporated into several yeast backgrounds to use fluorescence to monitor interactions under varying iron levels.
Advancing Professional Development Strategies For Chemistry And Biochemistry Undergraduates, Christian A. Brown
Advancing Professional Development Strategies For Chemistry And Biochemistry Undergraduates, Christian A. Brown
Senior Theses
In the Department of Chemistry and Biochemistry at the University of South Carolina (the Department), most curriculum-driven professional development is reserved for third- and fourth-year students. Classroom learning occurs at all levels, but the context comes late. Early exposure to career options and hands-on training is imperative for undergraduates to obtain maximum experiential learning. The purpose of this project is to advance the current professional development strategies of the Department, specifically targeting second- and third-year students. The study surveyed students' current involvement in professional development activities. The data on extracurricular experience and post-graduation interest was used to design a workshop …
Measuring The Impact: An Evaluation Of Communities In Harmony, Michael Carl Owens
Measuring The Impact: An Evaluation Of Communities In Harmony, Michael Carl Owens
Senior Theses
In the study reported below, a program evaluation was performed for the student organization Communities in Harmony, a music-based community service organization at the University of South Carolina that works with homeless and socioeconomically disadvantaged children and young adults. This organization is about to enter its fifth year and is in the process of transitioning between leadership teams at the time of publication. The purpose of this study is to evaluate the strengths and weaknesses of the organization so that it can continue to grow and effectively serve its target populations. This study has found that the organization excels at …