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Full-Text Articles in Molecular Biology
Riboregulation Of Bacterial Transposons, Michael J. Ellis
Riboregulation Of Bacterial Transposons, Michael J. Ellis
Electronic Thesis and Dissertation Repository
Bacterial transposons typically exist in a mutually beneficial relationship with the host cell. Limited transposition can benefit the host while also ensuring the survival of the element. An important component of this relationship is that transposition must be tightly regulated. In this thesis I explore ways that the host and transposon each control transposition in E. coli and provide evidence that a transposon can also control host gene expression in S. enterica Typhimurium. Post-transcriptional regulation with small non-coding RNAs (sRNA) has emerged as a key way that bacteria respond to stress and regulate many cellular processes. The RNA-binding protein Hfq …
Regulation Of The Bacterial Transposon Tn5, Crystal R. Mclellan
Regulation Of The Bacterial Transposon Tn5, Crystal R. Mclellan
Electronic Thesis and Dissertation Repository
Transposons play an integral role in bacterial adaptation, therefore it is important to understand the chemical steps and regulatory factors that govern their mobility. The properties that drive insertion of transposons into subsequent DNA sites have been studied for few elements. The transposon Tn5 in particular has few defined insertion sites and little is known about how its targets are selected. An objective of this thesis was to determine the molecular details of target insertion in Tn5 transposition in vitro. To this end, I assessed whether Tn5 could preferentially insert into short oligonucleotide substrates. I detected a Tn5 transpososome …