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- 5-methylcytosine, Activation induced deaminase, APOBEC3A, APOBEC3G, cytosine deamination, DNA demethylation (1)
- Amino acids (1)
- Aptamers (1)
- CD spectroscopy (1)
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- Competitive metalation (1)
- ESI mass spectrometry (1)
- Mechanism of metalation (1)
- Metal-induced folding (1)
- Metallothionein (1)
- NMR spectroscopy (1)
- Neurotransmitter receptors (1)
- Oligonucleotides (1)
- Protein folding (1)
- Proteins (1)
- RNA (1)
- Streptococcus (1)
- Supermetalated metallothionein (1)
- Zinc (1)
Articles 1 - 4 of 4
Full-Text Articles in Molecular Biology
Identification Of Persistent Long Range Interactions In GA95 And GB95 Through Thermal Unfolding Simulations, Milen Redai Tesfamariam
Identification Of Persistent Long Range Interactions In GA95 And GB95 Through Thermal Unfolding Simulations, Milen Redai Tesfamariam
Chemistry & Biochemistry Theses & Dissertations
For over five decades, different experiments have been performed to research how proteins attain their native three dimensional structures. However, the folding problem continues to be a puzzle in modern science. The design of two proteins that have maximal sequence identity but different folds and functions is one method that is being used to study the relationship between protein structure and amino acid sequence. In particular, mutant proteins of Streptococcus protein G, GA and GB, have 95% sequence identity and a 3a helix fold and β4/a fold, respectively. Molecular dynamics simulations of GA95 …
Structural Motifs Of Novel Metallothionein Proteins, Duncan E K Sutherland
Structural Motifs Of Novel Metallothionein Proteins, Duncan E K Sutherland
Electronic Thesis and Dissertation Repository
Metallothioneins (MT) are a family of small cysteine rich proteins, which have been implicated in toxic metal detoxification, protection against oxidative stress, and as a metallochaperone. The most well studied member of the family is the mammalian MT, which consists of two domains: a β-domain with 9 cysteine residues, which sequesters 3 Cd2+/Zn2+, and an α-domain with 11 cysteine residues, which sequesters 4 Cd2+/Zn2+. The exact functions of MT are unknown but must relate to its metalation status. Several areas that could lead to the assignment of function include 1) the determination …
Genetic And Biochemical Studies Of Human Apobec Family Of Proteins, Priyanga Wijesinghe
Genetic And Biochemical Studies Of Human Apobec Family Of Proteins, Priyanga Wijesinghe
Wayne State University Dissertations
The AID/APOBEC family of proteins in higher vertebrates converts cytosines in DNA or RNA into uracil. These proteins have essential roles in either innate immunity or adaptive immunity. Recently, AID has also been implicated in DNA demethylation in the context of early embryogenesis in mammals. This is partly based on the reported ability of AID to deaminate 5-methyl cytosine to thymine (5mC to T). I reexamined this proposed new role of AID (5mC deamination) with two members of the APOBEC family in a novel Escherichia coli based genetic system. My results confirmed that while all three enzymes are strong cytosine …
Inhibition Of Glutamate Receptors By Constructing Bipartite Rna Aptamers, Jeffrey Hebert
Inhibition Of Glutamate Receptors By Constructing Bipartite Rna Aptamers, Jeffrey Hebert
Legacy Theses & Dissertations (2009 - 2024)
The relationship of excessive activity of AMPA-type glutamate receptors, and cell death, has long provided researchers a means of investigating neurodegenerative disorders, such as Parkinson's Disease (PD) and amyotrophic lateral sclerosis (ALS). Inhibitors of AMPA receptor channels, including chemical and nucleic acid molecules such as RNA aptamers, have served as potential therapeutic agents and treatment of neurodegenerative disorders. In this study, building bipartite aptamers to enhance inhibitory potency, as compared with a monomeric aptamer of AMPA receptor, is described. An enhanced potency is due, at least in part; to the proximity effect in bipartite structures or binding of a monomeric …