Molecular Biology Commons^™

The Filament-Forming Protein Pil1 Assembles Linear Eisosomes In Fission Yeast, Ruth Kabeche, Suzanne Baldissard, John Hammond, Louisa Howard, James B. Moseley

Dartmouth Scholarship

The cortical cytoskeleton mediates a range of cellular activities such as endocytosis, cell motility, and the maintenance of cell rigidity. Traditional polymers, including actin, microtubules, and septins, contribute to the cortical cytoskeleton, but additional filament systems may also exist. In yeast cells, cortical structures called eisosomes generate specialized domains termed MCCs to cluster specific proteins at sites of membrane invaginations. Here we show that the core eisosome protein Pil1 forms linear cortical filaments in fission yeast cells and that purified Pil1 assembles into filaments in vitro. In cells, Pil1 cortical filaments are excluded from regions of cell growth and are …