Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Genes (2)
- Rna (2)
- Acids (1)
- Bayesian inference (1)
- Biocatalysis (1)
-
- Biochemistry (1)
- Biotinylation (1)
- Cdc (1)
- Cell biology (1)
- Cell cycle (1)
- Chlorophyll binding proteins (1)
- Chromatin immunoprecipitation (1)
- Down-regulation (1)
- Dyneins (1)
- E2f transcription factors (1)
- Ecosystem (1)
- Forkhead box protein m1 (1)
- Forkhead transcription factors (1)
- Foxm1 protein (1)
- Gene expression (1)
- Gene expression profiling (1)
- Gene expression regulation (1)
- Genetic (1)
- Glutamate dehydrogenase (1)
- Heat-shock proteins (1)
- Hela cells (1)
- Histones (1)
- Homo sapiens (1)
- Human (1)
- Humans (1)
Articles 1 - 4 of 4
Full-Text Articles in Molecular Biology
Gene Expression Studies For The Analysis Of Domoic Acid Production In The Marine Diatom Pseudo-Nitzschia Multiseries, Katie Boissonneault, Brooks M. Henningsen, Stephen S. Bates, Deborah L. Robertson, Sean Milton, Jerry Pelletier, Deborah A. Hogan, David E. Housman
Gene Expression Studies For The Analysis Of Domoic Acid Production In The Marine Diatom Pseudo-Nitzschia Multiseries, Katie Boissonneault, Brooks M. Henningsen, Stephen S. Bates, Deborah L. Robertson, Sean Milton, Jerry Pelletier, Deborah A. Hogan, David E. Housman
Dartmouth Scholarship
Pseudo-nitzschia multiseries Hasle (Hasle) (Ps-n) is distinctive among the ecologically important marine diatoms because it produces the neurotoxin domoic acid. Although the biology of Ps-n has been investigated intensely, the characterization of the genes and biochemical pathways leading to domoic acid biosynthesis has been limited. To identify transcripts whose levels correlate with domoic acid production, we analyzed Ps-n under conditions of high and low domoic acid production by cDNA microarray technology and reverse-transcription quantitative PCR (RT-qPCR) methods. Our goals included identifying and validating robust reference genes for Ps-n RNA expression analysis under these conditions.
Identification Of Cell Cycle–Regulated Genes Periodically Expressed In U2os Cells And Their Regulation By Foxm1 And E2f Transcription Factors, Gavin D. Grant, Lionel Brooks Iii, Xiaoyang Zhang, J. Matthew Mahoney, Viktor Martyanov, Tammara A. Wood, Gavin Sherlock, Chao Cheng, Michael L. Whitfield
Identification Of Cell Cycle–Regulated Genes Periodically Expressed In U2os Cells And Their Regulation By Foxm1 And E2f Transcription Factors, Gavin D. Grant, Lionel Brooks Iii, Xiaoyang Zhang, J. Matthew Mahoney, Viktor Martyanov, Tammara A. Wood, Gavin Sherlock, Chao Cheng, Michael L. Whitfield
Dartmouth Scholarship
We identify the cell cycle–regulated mRNA transcripts genome-wide in the osteosarcoma-derived U2OS cell line. This results in 2140 transcripts mapping to 1871 unique cell cycle–regulated genes that show periodic oscillations across multiple synchronous cell cycles. We identify genomic loci bound by the G2/M transcription factor FOXM1 by chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) and associate these with cell cycle–regulated genes. FOXM1 is bound to cell cycle–regulated genes with peak expression in both S phase and G2/M phases. We show that ChIP-seq genomic loci are responsive to FOXM1 using a real-time luciferase assay in live cells, showing that FOXM1 strongly …
The Tethering Complex Hops Catalyzes Assembly Of The Soluble Snare Vam7 Into Fusogenic Trans-Snare Complexes, Michael Zick, William Wickner
The Tethering Complex Hops Catalyzes Assembly Of The Soluble Snare Vam7 Into Fusogenic Trans-Snare Complexes, Michael Zick, William Wickner
Dartmouth Scholarship
The fusion of yeast vacuolar membranes depends on the disassembly of cis–soluble N-ethylmaleimide–sensitive factor attachment protein receptor (SNARE) complexes and the subsequent reassembly of new SNARE complexes in trans. The disassembly of cis-SNARE complexes by Sec17/Sec18p releases the soluble SNARE Vam7p from vacuolar membranes. Consequently, Vam7p needs to be recruited to the membrane at future sites of fusion to allow the formation of trans-SNARE complexes. The multisubunit tethering homotypic fusion and vacuole protein sorting (HOPS) complex, which is essential for the fusion of vacuolar membranes, was previously shown to have direct affinity for Vam7p. The …
Bayesian Reconstruction Of P(R) Directly From Two-Dimensional Detector Images Via A Markov Chain Monte Carlo Method, Sudeshna Paul, Alan M. Friedman, Chris Bailey-Kellogg, Bruce Craig
Bayesian Reconstruction Of P(R) Directly From Two-Dimensional Detector Images Via A Markov Chain Monte Carlo Method, Sudeshna Paul, Alan M. Friedman, Chris Bailey-Kellogg, Bruce Craig
Dartmouth Scholarship
The interatomic distance distribution, P(r), is a valuable tool for evaluating the structure of a molecule in solution and represents the maximum structural information that can be derived from solution scattering data without further assumptions. Most current instrumentation for scattering experiments (typically CCD detectors) generates a finely pixelated two-dimensional image. In continuation of the standard practice with earlier one-dimensional detectors, these images are typically reduced to a one-dimensional profile of scattering intensities, I(q), by circular averaging of the two-dimensional image. Indirect Fourier transformation methods are then used to reconstruct P(r) from …