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Full-Text Articles in Molecular Biology

Role And Regulation Of Myosin Iia In Natural Killer Cell Cytotoxicity, Keri Sanborn Aug 2011

Role And Regulation Of Myosin Iia In Natural Killer Cell Cytotoxicity, Keri Sanborn

Publicly Accessible Penn Dissertations

Natural killer (NK) cells are lymphocytes of the innate immune system that provide defense by directing their cytotoxic activity against virally infected and tumorigenic cells. Target cell lysis requires directed secretion of lytic granule contents, including pore-forming perforin and apoptosis-inducing granzymes, at the contact site. During the formation of a mature immunological synapse (IS) with a target cell, an NK cell polymerizes filamentous actin (F-actin) at the contact site, which may serve as a barrier to secretion of lytic granules for cytotoxicity. An actin-based motor protein, nonmuscle myosin IIA, has been demonstrated to be required for NK cell cytotoxic activity ...


Utilizing Genetically Engineered Mouse Models Of Pancreatic Cancer: Evaluating The Role Of Cathepsin B And The Efficacy Of Farnesyl Thiosalicylic Acid, Aarthi Gopinathan Dec 2010

Utilizing Genetically Engineered Mouse Models Of Pancreatic Cancer: Evaluating The Role Of Cathepsin B And The Efficacy Of Farnesyl Thiosalicylic Acid, Aarthi Gopinathan

Publicly Accessible Penn Dissertations

I have utilized genetically engineered mouse models of pancreatic cancer to identify a potential new therapeutic target, and to test the efficacy of a putative ras inhibitor. In the first part, I show that cathepsin B is upregulated during disease progression in the mouse pancreas, as is overall cathepsin activity. Loss of cathepsin B decreases preinvasive disease burden and imparts a significant survival benefit, with a consistent decrease in proliferation. In addition, lack of cathepsin B also decreases the burden of liver metastasis. Phospho-Erk localization appears to be affected by cathepsin B loss, which may account for the defect in ...