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Full-Text Articles in Molecular Biology
Heat Shock Protein 101 Effects In A. Thaliana: Genetic Variation, Fitness And Pleiotropy In Controlled Temperature Conditions., S. J. Tonsor, C. Scott, I. Boumaza, Elizabeth Vierling
Heat Shock Protein 101 Effects In A. Thaliana: Genetic Variation, Fitness And Pleiotropy In Controlled Temperature Conditions., S. J. Tonsor, C. Scott, I. Boumaza, Elizabeth Vierling
Elizabeth Vierling
The Hsp100/ClpB heat shock protein family is ancient and required for high temperature survival, but natural variation in expression and its phenotypic effects is unexplored in plants. In controlled environment experiments, we examined the effects of variation in the Arabidopsis cytosolic AtHsp101 (hereafter Hsp101). Ten wild-collected ecotypes differed in Hsp101 expression responses across a 22 to 40 degrees C gradient. Genotypes from low latitudes expressed the least Hsp101. We tested fitness and pleiotropic consequences of varying Hsp101 expression in 'control' vs. mild thermal stress treatments (15/25 degrees C D/N vs. 15/25 degrees D/N plus 3 h at 35 degrees C …
Modulation Of Nitrosative Stress By S-Nitrosoglutathione Reductase Is Critical For Thermotolerance And Plant Growth In Arabidopsis, Ung Lee, Chris Wie, Bernadette O. Fernandez, Martin Feelisch, Elizabeth Vierling
Modulation Of Nitrosative Stress By S-Nitrosoglutathione Reductase Is Critical For Thermotolerance And Plant Growth In Arabidopsis, Ung Lee, Chris Wie, Bernadette O. Fernandez, Martin Feelisch, Elizabeth Vierling
Elizabeth Vierling
Nitric oxide (NO) is a key signaling molecule in plants. This analysis of Arabidopsis thaliana HOT5 (sensitive to hot temperatures), which is required for thermotolerance, uncovers a role of NO in thermotolerance and plant development. HOT5 encodes S-nitrosoglutathione reductase (GSNOR), which metabolizes the NO adduct S-nitrosoglutathione. Two hot5 missense alleles and two T-DNA insertion, protein null alleles were characterized. The missense alleles cannot acclimate to heat as dark-grown seedlings but grow normally and can heat-acclimate in the light. The null alleles cannot heat-acclimate as light-grown plants and have other phenotypes, including failure to grow on nutrient plates, increased reproductive shoots, …
Core Genome Responses Involved In Acclimation To High Temperature, Jane Larkindale, Elizabeth Vierling
Core Genome Responses Involved In Acclimation To High Temperature, Jane Larkindale, Elizabeth Vierling
Elizabeth Vierling
Plants can acclimate rapidly to environmental conditions, including high temperatures. To identify molecular events important for acquired thermotolerance, we compared viability and transcript profiles of Arabidopsis thaliana treated to severe heat stress (45°C) without acclimation or following two different acclimation treatments. Notably, a gradual increase to 45°C (22°C to 45°C over 6 h) led to higher survival and to more and higher-fold transcript changes than a step-wise acclimation (90 min at 38°C plus 120 min at 22°C before 45°C). There were significant differences in the total spectrum of transcript changes in the two treatments, but core components of heat acclimation …