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Full-Text Articles in Molecular Biology
Cold-Temperature Adaptation Of Muscle Creatine Kinase From An Antartic Teleost (Chaenocephalus Aceratus), Paul Winnard Jr.
Cold-Temperature Adaptation Of Muscle Creatine Kinase From An Antartic Teleost (Chaenocephalus Aceratus), Paul Winnard Jr.
Electronic Theses and Dissertations
The white muscle of Chuenocephulus aceratus, an Antarctic teleost of the Channicthyidae family, has a compromised glycoiytic capacity and this fish cannot depend on glycolysis for rapid ATP generation For C. aceratus, creatine kinase (CK) and phosphocreatine (PCr) reserves comprise the metabolic pathway that may supplement and overcome this deficiency in energy transduction. Two conditions, low glycolytic capacity and evolution in a chronically cold habitat (-1.86°C), give us reason to believe that C. aceratus muscle CK (MMCK) has been subjected to strong selective pressure. Thus, the hypothesis of this thesis is that MMCK fiom C. aceratus white muscle …
Detection Of Insulin Receptor, Epidermal Growth Factor Receptor, And Interleukin-6 On Individual Mouse Embryos By Immuno-Polymerase Chain Reaction, Kun Xu
Electronic Theses and Dissertations
Three series of experiments were conducted to: 1) optimize the conditions for the production of pUC19 plasmid and of biotinylated pUC19 fragments; 2) optimize the conditions for the production of protein A-streptavidin chimera (chimeric protein); and 3) detect soluble antigens [bovine serum albumin (BSA) and interleukin-6 (IL-6)] and membrane-bound antigens [insulin receptor and epidermal growth factor receptor (EGFr)] of mouse embryos by immuno-polymerase chain reaction (I-PCR). The first experimental series, which included bacterial culture, chimeric protein purification, and chimeric protein functional experiments, was performed to investigate the effects of IPTG (isopropyl-β-D-thiogalactopyranoside) induction time and temperature, bacterial culture medium, and protein …
Quinic Acid-Mediated Induction Of Hypovirulence And A Hypovirulence-Associated Double-Stranded Rna In Rhizoctonia Solani, Chunyu Liu
Electronic Theses and Dissertations
This study is a part of a project focused on the relationship between dsRNA and hypovirulence in R. solani. Hypovirulence refers to a condition in which a pathogen has a drastically reduced ability to cause disease. Rhizoctonia solani is a soil-borne pathogen causing diseases in numerous plants. Phenyl acetic acid (PAA), a metabolite of phenylalanine, causes Rhizoctonia disease symptoms on potato in the absence of the pathogen itself. The amount of PAA produced by a hypovirulent isolate is 10% of that produced by virulent isolates. A 3.6 kb dsRNA (M2) has been shown to be associated with hypovirulence in …