Molecular Biology Commons^™

Copy Number Variation In Archival Melanoma Biopsies Versus Benign Melanocytic Lesions, Ahmed Mahas, Keerti Potluri, Michael N. Kent, Sameep Naik, Michael P. Markey

Biochemistry and Molecular Biology Faculty Publications

BACKGROUND: Skin melanocytes can give rise to benign and malignant neoplasms. Discrimination of an early melanoma from an unusual/atypical benign nevus can represent a significant challenge. However, previous studies have shown that in contrast to benign nevi, melanoma demonstrates pervasive chromosomal aberrations. OBJECTIVE: This substantial difference between melanoma and benign nevi can be exploited to discriminate between melanoma and benign nevi. METHODS: Array-comparative genomic hybridization (aCGH) is an approach that can be used on DNA extracted from formalin-fixed paraffin-embedded (FFPE) tissues to assess the entire genome for the presence of changes in DNA copy number. In this study, high resolution, …