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Purification Of Recombinant Δ Np63 Α And Characterization Of Peptide Binding, Amal Abdulah Albati
Purification Of Recombinant Δ Np63 Α And Characterization Of Peptide Binding, Amal Abdulah Albati
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δ NP63α, the primary p63 isoform of the p53 transcription factor family, is a proto-oncogene implicated in non-melanoma skin cancers. Expressed in the basal layer of the epidermis, δ NP63α promotes cell survival and proliferation. Inhibition of this protein could potentially be beneficial in non-melanoma skin cancer patients. The first goal of this project was the purification of recombinant δ NP63α in Escherichia Coli. Recombinant δ NP63α was expressed as GST-δ NP63α followed by GST cleavage using GST trap affinity column chromatography yielding pure δ NP63α The second objective of this project was to test the binding capabilities of peptides …