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Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana, Prince Kudjoe Zogli
Analysis Of Function Of The Son1-Interacting Protein, Lnk2 In Arabidopsis Thaliana, Prince Kudjoe Zogli
Graduate College Dissertations and Theses
The Arabidopsis SON1 F-box protein was implicated in regulating a pathogen defense pathway, but its exact function in wild-type plants is unknown. As an F-box protein it was predicted that SON1 would assembles into a SON1-SCF ubiquitin ligase complex that recruits specific plant defense-related proteins for proteolysis. The yeast 2-hybrid assay was used to screen for potential substrates for a putative SON1-SCF ligase, leading to the identification of Arabidopsis LNK2 as a SON1-binding factor.
Comprehensive protein-protein interaction analysis has shown that the binding of SON1 to LNK2 protein is specific, because closely related, full-length Arabidopsis F-box proteins do not interact …