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Articles 1 - 9 of 9
Full-Text Articles in Molecular Biology
The Estradiol-Induced Transcriptome Of The Female Mouse Anteroventral Periventricular Nucleus: More Than Just A Kiss, Leah K. Aggison
The Estradiol-Induced Transcriptome Of The Female Mouse Anteroventral Periventricular Nucleus: More Than Just A Kiss, Leah K. Aggison
Doctoral Dissertations
Estradiol (E2) is critical in the reproductive mechanisms of mammals. In female rodents E2 acts through the neurons of the anteroventral periventricular nucleus (AVPV) to exert neuroendocrine control over ovulation, via synaptic activation of the gonadotropin releasing hormone (GnRH) neurons. The neurocircuitry of the AVPV is complex, receiving input from the suprachiasmatic nucleus and ventral premammillary nucleus and the as well as projecting to organum vasculosum of lamina terminalis and the arcuate. This suggests a broader role for the AVPV as a center of multisignal-integration in regards to ovulation. I used full genome expression microarrays to assess …
Expression And Purification Of Human Lysosomal Β-Galactosidase From Pichia Pastoris, Sarah E. Tarullo
Expression And Purification Of Human Lysosomal Β-Galactosidase From Pichia Pastoris, Sarah E. Tarullo
Masters Theses
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported to the lysosome. However, in diseased cells, these lysosomal enzymes are either absent or malfunctioning, causing macromolecular substrates to accumulate, becoming toxic to the cell. Over fifty lysosomal storage diseases have been identified, collectively occurring in one out of 7,700 live births. We investigated the lysosomal enzyme β-galactosidase (β-gal). In order to study the biochemistry and enzymology of this protein a robust expression system was needed. The GLB1 gene has been inserted into …
Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo
Sequence Analysis Of Maize Yellow Stripe3 Candidate Genes, Dennis B. Depaolo
Masters Theses
The work presented here focuses on the molecular mechanism of phytosiderophore secretion in graminaceous plants. In maize, yellow stripe3 (ys3) is a mutant that is deficient in its ability to secrete iron-chelating compounds of the mugineic acid family known as phytosiderophores. Phytosiderophores are specific to grasses and are used for the acquisition of iron. Genetic linkage mapping of the ys3 locus lead to a region of interest on chromosome 3 defined by marker UMC1773. The sequence of eleven candidate genes (GRMZM2G390345, GRMZM2G390374, GRMZM2G342821, GRMZM5G800764, GRMZM2G502560, GRMZM5G849435, GRMZM2G105766, GRMZM5G876835, GRMZM2G036976, GRMZM2G502563, miR167g) revealed several small deletions …
Designing A Pore-Forming Toxin Cytolysin A (Clya) Specific To Target Cancer Cells, Alzira Rocheteau Avelino
Designing A Pore-Forming Toxin Cytolysin A (Clya) Specific To Target Cancer Cells, Alzira Rocheteau Avelino
Masters Theses
Cytolysin A (ClyA) is a member of a class of proteins called pore-forming toxins (PFTs). ClyA is secreted by Gram-negative bacteria, and it attacks a number of mammalian cells by inserting into and forming channels within the cell membrane (Oscarsson J et al., 1999). It has been suggested that ClyA binds to cholesterol (Oscarsson J et al., 1999) and thus can insert into the membranes of many different cell types of eukaryotic origin. In our studies we propose to engineer a ClyA protein that can only attack a small subset of cell types. We propose to engineer ClyA that can …
Novel Strategies To Modulate Synaptic Communication And Investigate The Role Of Hdac6 In Alzheimer’S Disease, Kathryne A. Medeiros
Novel Strategies To Modulate Synaptic Communication And Investigate The Role Of Hdac6 In Alzheimer’S Disease, Kathryne A. Medeiros
Doctoral Dissertations
Neuronal communication is mediated by chemical signaling at the synapse. The underlying molecular mechanisms of learning and memory are poorly understood. Very few tools are available to study how memories are formed in the mammalian brain. This dissertation focuses on developing novel strategies to study neural activity. Here we develop and use a chemical-genetic approach to enable target-specific photocontrol of inhibitory synaptic neurotransmission of GABAA receptor subtypes. The tools developed here selectively photocontrolled GABAA receptor subtypes. This enabled the investigation of the functional role these receptor subtypes have in inhibitory synaptic neurotransmission. This dissertation also focuses on identifying …
Engineering Probes To Detect Cholesterol Accessibility On Membranes Using Perfringolysin O, Benjamin B. Johnson
Engineering Probes To Detect Cholesterol Accessibility On Membranes Using Perfringolysin O, Benjamin B. Johnson
Doctoral Dissertations
Cholesterol is an essential component of mammalian cell membranes and it is important to regulate the structure and function of lipid bilayers. Changes in cholesterol levels are involved in many physiological and pathological events such as the formation of arterial plaques, viral entry into cells, sperm capacitation, and receptor organization. Determination of cholesterol trafficking and distribution is essential for understanding how cells regulate cholesterol. A key factor in the regulation of cholesterol is cholesterol accessibility. Through it interactions in the membrane, cholesterol is sequestered below the surface of the membrane. Based on the composition of the membrane, a certain amount …
Effect Of A 10 Day Decrease In Physical Activity On Circulating Angiogenic Cells, Gayatri Guhanarayan
Effect Of A 10 Day Decrease In Physical Activity On Circulating Angiogenic Cells, Gayatri Guhanarayan
Masters Theses 1911 - February 2014
Circulating angiogenic cells (CACs) are early predictors of cardiovascular health and are inversely proportional to related outcomes. Increased number and function of CACs is seen in healthy individuals compared with individuals with cardiovascular disease (CVD). Exercise increases CAC number and function in CVD populations, through a nitric oxide-mediated mechanism. Inactivity is a growing concern in industrialized nations; it is an independent risk factor for CVD and is linked to increased mortality. The purpose of this study was to understand the effect of reduced physical activity (rPA) on two CAC populations (CFU-Hill and CD34+) in highly active individuals. We …
Genetic Modification Of Plants, Alice Cheung, Hen-Ming Wu
Genetic Modification Of Plants, Alice Cheung, Hen-Ming Wu
Science and Engineering Saturday Seminars
No abstract provided.
Activation-Triggered Subunit Exchange Between Camkii Holoenzymes Facilitates The Spread Of Kinase Activity, Margaret M. Stratton, I H. Lee, M Bhattacharyya, S M. Christensen, L H. Chao, H Schulman, J T. Groves, J Kuriyan
Activation-Triggered Subunit Exchange Between Camkii Holoenzymes Facilitates The Spread Of Kinase Activity, Margaret M. Stratton, I H. Lee, M Bhattacharyya, S M. Christensen, L H. Chao, H Schulman, J T. Groves, J Kuriyan
Biochemistry & Molecular Biology Department Faculty Publication Series
The activation of the dodecameric Ca2+/calmodulin dependent kinase II (CaMKII) holoenzyme is critical for memory formation. We now report that CaMKII has a remarkable property, which is that activation of the holoenzyme triggers the exchange of subunits between holoenzymes, including unactivated ones, enabling the calcium-independent phosphorylation of new subunits. We show, using a single-molecule TIRF microscopy technique, that the exchange process is triggered by the activation of CaMKII, and that exchange is modulated by phosphorylation of two residues in the calmodulin-binding segment, Thr 305 and Thr 306. Based on these results, and on the analysis of molecular dynamics simulations, we …