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Full-Text Articles in Molecular Biology

Plants, Pipettes And Pcr, Elizabeth Vierling Jan 2022

Plants, Pipettes And Pcr, Elizabeth Vierling

Science and Engineering Saturday Seminars

Plants are amazing organisms that provide us with food, building materials, the pleasure of gardens, as well as providing the foundation of critical world ecosystems. Although they may look like they are just stuck in one place and doing not more than growing, they have many, many complex ways in which they respond to the environment. The goal of this STEM Ed session will be to discuss ways that plants can respond to the environment with hands on exercises and exploration of possible classroom activities. Participants will engage in state-of-the art methods of testing plant DNA composition using the polymerase …


Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal Jan 2009

Functional Analysis Of Receptor-Like Kinases In Pollen-Pistil Interactions In Arabidopsis Thaliana, Mini Aggarwal

Masters Theses 1911 - February 2014

Rac/Rop GTPases are molecular switches in plants that control the growth of polarized cells such as pollen tubes and root hairs, differentiation, development, actin dynamics, production of reactive oxygen species (ROS) and disease resistance. These small GTPases are activated by guanine nucleotide exchange factors (GEFs) that replace GDP for GTP and are referred to as RopGEFs in plants. To identify upstream components of the RopGEF regulated signaling pathways, GEF1 from Arabidopsis thaliana was used as a bait to screen a seedling cDNA library in a yeast two-hybrid system. This yielded members of a small family of the Catharanthus roseus Receptor-like …