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Full-Text Articles in Molecular Biology
Pdip46 (Dna Polymerase Delta Interacting Protein 46) Is An Activating Factor For Human Dna Polymerase Delta, Xiaoxiao Wang, Sufang Zhang, Rong Zheng, Fu Yue, Szu Hua Sharon Lin, Amal Rahmeh, Ernest Y C Lee, Zhongtao Zhang, Marietta Y W T Lee
Pdip46 (Dna Polymerase Delta Interacting Protein 46) Is An Activating Factor For Human Dna Polymerase Delta, Xiaoxiao Wang, Sufang Zhang, Rong Zheng, Fu Yue, Szu Hua Sharon Lin, Amal Rahmeh, Ernest Y C Lee, Zhongtao Zhang, Marietta Y W T Lee
NYMC Faculty Publications
PDIP46 (SKAR, POLDIP3) was discovered through its interaction with the p50 subunit of human DNA polymerase δ (Pol δ). Its functions in DNA replication are unknown. PDIP46 associates with Pol δ in cell extracts both by immunochemical and protein separation methods, as well as by ChIP analyses. PDIP46 also interacts with PCNA via multiple copies of a novel PCNA binding motif, the APIMs (AlkB homologue-2 PCNA-Interacting Motif). Sites for both p50 and PCNA binding were mapped to the N-terminal region containing the APIMs. Functional assays for the effects of PDIP46 on Pol δ activity on singly primed ssM13 DNA templates …