Multiple Puf Proteins Regulate The Stability Of Ribosome Biogenesis Transcripts, Anthony D. Fischer, Wendy M. Olivas
Sep 2018
Multiple Puf Proteins Regulate The Stability Of Ribosome Biogenesis Transcripts, Anthony D. Fischer, Wendy M. Olivas
Wendy Olivas
Cells must make careful use of the resources available to them. A key area of cellular regulation involves the biogenesis of ribosomes. Transcriptional regulation of ribosome biogenesis factor genes through alterations in histone acetylation has been well studied. This work identifies a post-transcriptional mechanism of ribosome biogenesis regulation by Puf protein control of mRNA stability. Puf proteins are eukaryotic mRNA binding proteins that play regulatory roles in mRNA degradation and translation via association with specific conserved elements in the 3ʹ untranslated region (UTR) of target mRNAs and with degradation and translation factors. We demonstrate that several ribosome biogenesis factor mRNAs …
Conditional Regulation Of Puf1p, Puf4p, And Puf5p Activity Alters Yhb1 Mrna Stability For A Rapid Response To Toxic Nitric Oxide Stress In Yeast, Joseph Russo, Wendy M. Olivas
Mar 2015
Conditional Regulation Of Puf1p, Puf4p, And Puf5p Activity Alters Yhb1 Mrna Stability For A Rapid Response To Toxic Nitric Oxide Stress In Yeast, Joseph Russo, Wendy M. Olivas
Wendy Olivas
Puf proteins regulate mRNA degradation and translation through interactions with 3' untranslated regions (UTRs). Such regulation provides an efficient method to rapidly alter protein production during cellular stress. YHB1 encodes the only protein to detoxify nitric oxide in yeast. Here we show that YHB1 mRNA is destabilized by Puf1p, Puf4p, and Puf5p through two overlapping Puf recognition elements (PREs) in the YHB1 3' UTR. Overexpression of any of the three Pufs is sufficient to fully rescue wild-type decay in the absence of other Pufs, and overexpression of Puf4p or Puf5p can enhance the rate of wild-type decay. YHB1 mRNA decay …
Carbon Source-Dependent Alteration Of Puf3p Activity Mediates Rapid Changes In The Stabilities Of Mrnas Involved In Mitochondrial Function, Melanie A. Miller, Joseph Russo, Anthony D. Fischer, Florencia A. Lopez Leban, Wendy M. Olivas
Mar 2014
Carbon Source-Dependent Alteration Of Puf3p Activity Mediates Rapid Changes In The Stabilities Of Mrnas Involved In Mitochondrial Function, Melanie A. Miller, Joseph Russo, Anthony D. Fischer, Florencia A. Lopez Leban, Wendy M. Olivas
Wendy Olivas
The Puf family of RNA-binding proteins regulates gene expression primarily by interacting with the 3′ untranslated region (3′ UTR) of targeted mRNAs and inhibiting translation and/or stimulating decay. Physical association and computational analyses of yeast Puf3p identified >150 potential mRNA targets involved in mitochondrial function. However, only COX17 has been established as a target of Puf3p-mediated deadenylation and decapping. We have identified 10 new targets that are rapidly degraded in a Puf3p-dependent manner. We also observed changes in Puf3p activity in response to environmental conditions. Puf3p promotes rapid degradation of mRNA targets in the fermentable carbon source dextrose. However, Puf3p-mediated …
