Molecular Biology Commons^™

This article, which includes novel unpublished data along with commentary and analysis,

focuses on approaches to link transcriptomic, proteomic, and peptidomic datasets mined from

brain tissue to the original locations within the brain that they are derived from using digital atlas

mapping techniques. We use, as an example, the transcriptomic, proteomic and peptidomic

analyses conducted in the mammalian hypothalamus. Following a brief historical overview, we

highlight studies that have mined biochemical and molecular information from the hypothalamus

and then lay out a strategy for how these data can be linked spatially to the mapped locations in a

canonical brain atlas …

The rostral ventrolateral medulla (RVLM) is an area of the brain stem that contains diverse neural substrates that are involved in systems critical for physiological function. There is evidence that aging affects some neural substrates within the RVLM, although age-related changes in RVLM molecular mechanisms are not well established. The goal of the present study was to characterize the transcriptomic profile of the aging RVLM and to test the hypothesis that aging is associated with altered gene expression in the RVLM, with an emphasis on immune system associated gene transcripts. RVLM tissue punches from young, middle-aged, and aged F344 rats …