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Constructing A Vector For Over Expressing Nitrobenzene Dioxygenase In E. Coli, Paul Davis
Constructing A Vector For Over Expressing Nitrobenzene Dioxygenase In E. Coli, Paul Davis
Honors Student Works
Due to extensive use of nitroaromatic compounds in the production of dyes, pesticides, explosives, and their harmful effects on the environment, studying the enzyme mechanisms of bacteria capable of utilizing these compounds as nutrient sources have become a recent field of development. One example, the Comamonas sp. strain JS765, grows on nitrobenzene as its sole carbon, nitrogen and energy source. This bacterium contains a nitrobenzene dioxygenase enzyme system that incorporates O2 into the nitrobenzene ring to form catechol and nitrite. In this project, the genes that code for the dioxygenase enzyme were cloned and then transformed into an expression vector …