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Full-Text Articles in Molecular Biology
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Searching For Potential Binding Partners Of Arabidopsis Β-Amylase2 Using Yeast 2-Hybridization, Megan E. Hines
Senior Honors Projects, 2010-2019
BAM2 is a chloroplast-targeted member of the β-amylase gene family that currently has an unknown function in starch hydrolysis. Previous research indicated that BAM2 did not have significant catalytic activity, but, because the gene is highly conserved and there is a starch-excess phenotype in older plants lacking BAM2, it was hypothesized that BAM2 may instead have a regulatory function. Many regulatory proteins function by interacting with other proteins, so we wanted to test for potential protein binding partners for BAM2 using the yeast two-hybrid system. A plasmid containing the BAM2 gene from Arabidopsis thaliana was co-transformed into Y190-strain yeast ( …
Identification Of N-Acylethanolamine Hydrolyzing Enzyme In Solanum Lycopersicum, Derek A. Stuffle
Identification Of N-Acylethanolamine Hydrolyzing Enzyme In Solanum Lycopersicum, Derek A. Stuffle
Undergraduate Honors Theses
N-acylethanolamines (NAEs) are fatty acid derivatives that occur naturally in plant and animal systems. In mammals, they regulate physiological functions, including neurotransmission, immune responses, vasodilation, embryo development and implantation, feeding behavior, and cell proliferation. NAEs are metabolized by fatty acid amide hydrolase (FAAH), which belongs to the amidase signature family. It is hypothesized that putative FAAH functions as the catalyst in the metabolism of N-acylethanolamine in tomato plants. To test the hypothesis, FAAH protein homologs were identified in tomato via in silico analysis. Among the six homologs identified, FAAH1 and FAAH2 were selected for further validation. This study …