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Full-Text Articles in Molecular Biology

Identification Of Factors Involved In 18s Nonfunctional Ribosomal Rna Decay And A Method For Detecting 8-Oxoguanosine By Rna-Seq, Kelly A. Limoncelli Dec 2017

Identification Of Factors Involved In 18s Nonfunctional Ribosomal Rna Decay And A Method For Detecting 8-Oxoguanosine By Rna-Seq, Kelly A. Limoncelli

GSBS Dissertations and Theses

The translation of mRNA into functional proteins is essential for all life. In eukaryotes, aberrant RNAs containing sequence features that stall or severely slow down ribosomes are subject to translation-dependent quality control. Targets include mRNAs encoding a strong secondary structure (No-Go Decay; NGD) or stretches of positively-charged amino acids (Peptide-dependent Translation Arrest/Ribosome Quality Control; PDTA/RQC), mRNAs lacking an in-frame stop codon (Non-Stop Decay; NSD), or defective 18S rRNAs (18S Nonfunctional rRNA Decay; 18S NRD). Previous work from our lab showed that the S. cerevisiae NGD factors DOM34 and HBS1, and PDTA/RQC factor ASC1, all participate in the ...


The Essential Jil-1 Kinase Regulates Histone H3 Phosphorylation And Maintains Chromatin Structure In Drosophila , Weiguo Zhang Jan 2004

The Essential Jil-1 Kinase Regulates Histone H3 Phosphorylation And Maintains Chromatin Structure In Drosophila , Weiguo Zhang

Retrospective Theses and Dissertations

JIL-1 encodes a chromatin-associated tandem serine/threonine kinase in Drosophila melanogaster. JIL-1 predominantly associates with interbands on polytene chromosomes. It appears at higher levels on the X chromosome than on autosomes in males, and distributes about equally on all chromosomes in females. In addition, in males JIL-1 overlaps with the MSL (for male specific lethal) complex that specifically associates with the male X chromosome on which expression of most genes are upregulated.;We isolated JIL-1 mutants by inducing imprecise excision of an EP element inserted in JIL-1. We found that JIL-1 is required for viability of both females and males ...


Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang Jan 2003

Identification And Functional Analysis Of Thylakoid Membrane Proteome , Yingchun Wang

Retrospective Theses and Dissertations

Membrane proteins play crucial roles in many biological functions. Identities and functions of most membrane proteins remain to be revealed. New technological breakthroughs in proteomics together with the availability of genomic sequence information make it possible to study functions of membrane proteins on a genome-wide scale. We used a multidisciplinary approach combining biochemistry, genetics, proteomics and bioinformatics to study the functions of the thylakoid proteome of Synechocystis sp. PCC6803. The thylakoid membrane proteins were separated into peripheral and integral fractions and resolved into 2-D gels with different pH ranges. The protein spots in the 2-D gels were subjected to peptide ...


Oxidizing Side Of The Photosystem I , Jun Sun Jan 1999

Oxidizing Side Of The Photosystem I , Jun Sun

Retrospective Theses and Dissertations

Photosynthesis converts solar energy into the biological sources of energy for the life on our planet. Photosystem I (PSI) is one of the two reaction centers of oxygenic photosynthesis. PSI is a multiheteromeric membrane-protein complex that catalyzes light-driven electron transfer from plastocyanin or cytochrome c6 to ferredoxin. The PsaA and PsaB subunits form the heterodimeric core that harbors the primary electron donor P700. On the oxidizing side of the PSI complex, plastocyanin or cytochrome c6 donates electrons to the P700 reaction center. The objective of this dissertation is to identify elements of molecular recognition on the oxidizing side of PSI ...


Investigating The Mechanism Of Mrp/Plf Gene Expression By Bfgf , Alesia Marie Hruska-Hageman Jan 1998

Investigating The Mechanism Of Mrp/Plf Gene Expression By Bfgf , Alesia Marie Hruska-Hageman

Retrospective Theses and Dissertations

The mitogen-regulated protein/proliferin (mrp/ plf) genes belong to the prolactin/growth hormone gene superfamily and encode at least four closely related proteins. Identified functions of these proteins include stimulation of uterine proliferation and endothelial angiogenesis. In 3T3 cells, basic fibroblast growth factor (bFGF) stimulates the production of mrp/plf mRNAs with a resulting increase in the protein products. Although the three cloned mrp/ plf gene promoters (mrp3, plf42, and plf149 ) are over 97% identical in sequence, only mrp3 is transcriptionally activated by bFGF. We have identified a sequence in the mrp3 promoter, which we have named the "bFGF-responsive element ...


A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan Dec 1990

A Temperature-Sensitive Mutant Of Escherichia Coli Affected In The Alpha Subunit Of Rna Polymerase, Majid Mehrpouyan

Electronic Theses and Dissertations

A temperature-sensitive mutant of Escherichia coli affected in the alpha subunit of RNA polymerase has been investigated. Gene mapping and complementation experiments placed the mutation to temperature-sensitivity within the alpha operon at 72 min on the bacterial chromosome. The rate of RNA synthesis in vivo and the accumulation of ribosomal RNA were significantly reduced in the mutant at 44$\sp\circ$C. The thermostability at 44$\sp\circ$C of the purified holoenzyme from mutant cells was about 20% of that of the normal enzyme. Assays with T7 DNA as a template showed that the fraction of active enzyme competent ...