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Full-Text Articles in Molecular Biology
Chromatin Insulators: Master Regulators Of The Eukaryotic Genome, Todd Andrew Schoborg
Chromatin Insulators: Master Regulators Of The Eukaryotic Genome, Todd Andrew Schoborg
Doctoral Dissertations
Proper organization of the chromatin fiber within the three dimensional space of the eukaryotic nucleus relies on a number of DNA elements and their interacting proteins whose structural and functional consequences exert significant influence on genome behavior. Chromatin insulators are one such example, where it is thought that these elements assist in the formation of higher order chromatin loop structures by mediating long-range contacts between distant sites scattered throughout the genome. Such looping serves a dual role, helping to satisfy both the physical constraints needed to package the linear DNA polymer within the small volume of the nucleus while simultaneously …
Functional Analysis Of Corazonin And Its Receptor In Drosophila Melanogaster, Kai Sha
Functional Analysis Of Corazonin And Its Receptor In Drosophila Melanogaster, Kai Sha
Doctoral Dissertations
Corazonin (Crz) is an amidated undecapeptide originally isolated from the American cockroach. It has been shown to affect diverse physiological functions in a species-specific manner. However, the functionality of Crz in Drosophila melanogaster has not yet been determined. To gain insight into the role of Crz signaling in vivo, Crz and CrzR null alleles were obtained by transposable element mobilization. Flies carrying a deficiency uncovering Crz and pr-set7 loci were generated via P-element excision, and the latter was rescued by wild-type pr-set7 transgene. A mutation of Crz receptor (CrzR) was generated by Minos-element mobilization from …