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24 Hour Incubation Of Ovary Tissue In 17Α20Β-Dihyroxy-4-Pregnon-3-One Causes Bcl-2 And Pi3k-Iii Expression To Vary In Danio Rerio, Beth Onaga
Summer Research
The present study investigated the effects of increasing concentrations of progesterone on cell death by monitoring the expression of mRNAs specific for genes in the apoptosis and autophagy cell death pathway in Danio rerio ovary tissue using quantitative (q)PCR. Progesterone is used to regulate oocyte maturation in vivo; however, synthetic progesterone in oral contraceptives is found in the environment. Primers for Bcl-2 and Pi3K-III were used to investigate gene activity via mRNA produced. The results of the present study revealed that gene activity was variable as revealed by qPCR analysis. After 24 hour exposure to progesterone Bcl-2 and Pi3K-III were …