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Isolation Of Rat Adipose Tissue Mesenchymal Stem Cells For Differentiation Into Insulin-Producing Cells, Dina H. Kassem, Sarah A. Habib, Omar I. Badr, Mohamed M. Kamal
Isolation Of Rat Adipose Tissue Mesenchymal Stem Cells For Differentiation Into Insulin-Producing Cells, Dina H. Kassem, Sarah A. Habib, Omar I. Badr, Mohamed M. Kamal
Pharmacy
Mesenchymal stem cells (MSCs)-especially those isolated from adipose tissue (Ad-MSCs)-have gained special attention as a renewable, abundant source of stem cells that does not pose any ethical concerns. However, current methods to isolate Ad-MSCs are not standardized and employ complicated protocols that require special equipment. We isolated Ad-MSCs from the epididymal fat of Sprague-Dawley rats using a simple, reproducible method. The isolated Ad-MSCs usually appear within 3 days post isolation, as adherent cells display fibroblastic morphology. Those cells reach 80% confluency within 1 week of isolation. Afterward, at passage 3-5 (P3-5), a full characterization was carried out for the isolated …