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Full-Text Articles in Molecular Biology
Production, Purification And Crystallization Of Membrane Integrated Multimeric Bax, Adelbert Mark Villoso
Production, Purification And Crystallization Of Membrane Integrated Multimeric Bax, Adelbert Mark Villoso
UNLV Theses, Dissertations, Professional Papers, and Capstones
Apoptosis, or programmed cell death, is a vital process intimately involved in the embryonic development and homeostatic maintenance of all multicellular organisms. The committing step to apoptosis is regulated by a key protein, Bax, and its ability to integrate and form a pore structure at the outer mitochondrial membrane.
Unfortunately, the molecular details of apoptosis remain largely unclear due to the lack of structural data of integral membrane (IM) Bax. Experimental limitations of membrane protein production have slowed the pursuit of an IM-Bax structure simply because standard protocols for producing recombinant IM-Bax are inefficient in producing adequate quantities of IM-Bax …