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Characterization Of A Hypothetical Protein Critical For The Symbiotic Interaction Of Aeromonas Veronii And Hirudo Verbana, Kaitlin Vaughan
Characterization Of A Hypothetical Protein Critical For The Symbiotic Interaction Of Aeromonas Veronii And Hirudo Verbana, Kaitlin Vaughan
Honors Scholar Theses
The digestive tract symbiosis of the medicinal leech, Hirudo verbana, is a model system for studying the genes required for microbial colonization of digestive tracts, as H. verbana has only two species of bacteria that dominate the crop microbiota, Aeromonas veronii and a Rikenella-like bacterium. Signature-tagged mutagenesis (STM) of the A.
veronii strain, HM21R, revealed genes required for the colonization of the digestive tract. One of these mutants, JG573, has an interrupted gene that is predicted to encode a hypothetical protein. The region flanking the transposon insertion of this mutant was sequenced by primer walking.
Comparison of the flanking DNA …