Open Access. Powered by Scholars. Published by Universities.®
- Keyword
-
- DREAM (2)
- Alzheimer’s disease (1)
- Binding interface (1)
- Biomolecular interactions (1)
- Biophysics (1)
-
- CaBP (1)
- Calcium (1)
- Calcium binding proteins (1)
- Carbon nanotube based nanoporous membrane devices (1)
- Casenilin (1)
- Circular dichroism (1)
- Computational MD simulation (1)
- Conformational changes of protein (1)
- DNA supercoiling (1)
- DNA topoisomerases (1)
- Drug discovery (1)
- Drug resistance (1)
- Dynamics network analysis (1)
- EF-hand (1)
- Envelope protein (1)
- Finite element simulations (1)
- Fluorescence Spectroscopy (1)
- Fluorescence spectroscopy (1)
- Heme proteins (1)
- ITC (1)
- Isothermal titration calorimetry (1)
- KChIP (1)
- KChIP3 (1)
- Kv4.3 (1)
- Label-free methods (1)
Articles 1 - 4 of 4
Full-Text Articles in Molecular Biology
The Role Of Conformational Changes In Viral And Bacterial Protein Functions, Md Lokman Hossen
The Role Of Conformational Changes In Viral And Bacterial Protein Functions, Md Lokman Hossen
FIU Electronic Theses and Dissertations
Proteins do versatile work in cells. They require a cascade of structural changes to perform different tasks like binding to the other neighboring biomolecules, transporting small chemicals, activating a chemical reaction, etc. The structural conformations of proteins can be critical in changing their working ability. In this dissertation, I investigated the role of conformational changes of viral protein, e.g., spike and envelope protein of SARS-CoV-2, and bacterial protein, e.g., multidrug transporter and toxic extrusion protein- PfMATE from Pyrococcus furiosus. Also, I performed molecular docking-based drug screening targeting the E protein to suggest a set of drugs that can be repurposed …
Conformational Dynamics And Stability Associated With Magnesium Or Calcium Binding To Dream In The Regulation Of Interactions Between Dream And Dna Or Presenilins, Khoa Ngoc Pham
FIU Electronic Theses and Dissertations
Downstream regulatory element antagonist modulator (DREAM) is involved in various interactions with targets both inside and outside of the nucleus. In the cytoplasm, DREAM interacts with the C-terminal fragments of presenilins to facilitate the production of β-amyloid plaques in Alzheimer’s disease. In the nucleus, Ca2+ free DREAM directly binds to specific downstream regulatory elements of prodynorphin/c-fos gene to repress the gene transcription in pain modulation. These interactions are regulated by Ca2+ and/or Mg2+ association at the EF-hands in DREAM. Therefore, understanding the conformational dynamics and stability associated with Ca2+ and/or Mg2+ binding to DREAM …
Protein-Ligand Interactions And Allosteric Regulation Of Activity In Dream Protein, Walter G. Gonzalez
Protein-Ligand Interactions And Allosteric Regulation Of Activity In Dream Protein, Walter G. Gonzalez
FIU Electronic Theses and Dissertations
Downstream regulatory antagonist modulator (DREAM) is a calcium sensing protein that co-assembles with KV4 potassium channels to regulate ion currents as well as with DNA in the nucleus, where it regulates gene expression. The interaction of DREAM with A-type KV4 channels and DNA has been shown to regulate neuronal signaling, pain sensing, and memory retention. The role of DREAM in modulation of pain, onset of Alzheimer’s disease, and cardiac pacemaking has set this protein as a novel therapeutic target. Moreover, previous results have shown a Ca2+ dependent interaction between DREAM and KV4/DNA involving …
Multimode Analysis Of Nanoscale Biomolecular Interactions, Purushottam Babu Tiwari
Multimode Analysis Of Nanoscale Biomolecular Interactions, Purushottam Babu Tiwari
FIU Electronic Theses and Dissertations
Biomolecular interactions, including protein-protein, protein-DNA, and protein-ligand interactions, are of special importance in all biological systems. These interactions may occer during the loading of biomolecules to interfaces, the translocation of biomolecules through transmembrane protein pores, and the movement of biomolecules in a crowded intracellular environment. The molecular interaction of a protein with its binding partners is crucial in fundamental biological processes such as electron transfer, intracellular signal transmission and regulation, neuroprotective mechanisms, and regulation of DNA topology. In this dissertation, a customized surface plasmon resonance (SPR) has been optimized and new theoretical and label free experimental methods with related analytical …