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Biology

1993

Articles 1 - 2 of 2

Full-Text Articles in Molecular Biology

Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei Sep 1993

Myocardial Alpha-Thrombin Receptor Activation Induces Hypertrophy And Increases Atrial Natriuretic Factor Gene Expression, C C. Glembotski, C E. Irons, K A. Krown, S F. Murray, A B. Sprenkle, C A. Sei

Amy Sprenkle

The protease, a-thrombin (aTh), affects myocardial cell contractility, a feature common among agents that induce hypertrophy. However, it is not known whether cardiac myocytes possess aTh receptors (aTh-R), or if long term treatment with aTh can enhance growth and gene expression. In the present study primary neonatal rat ventricular myocytes expressed a 3.6-kilobase mRNA species that hybridized with a rat aTh-R-specific probe. After 48 h, aTh induced hypertrophy, sarcomeric organization, and enhanced atrial natriuretic factor (ANF) expression, all of which were blocked by the aTh-selective protease inhibitor, D-Phe-Pro-Argchloromethyl ketone. The aTh-R agonist peptide, SFLLRNPND, was a potent activator of ANF …

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Morfología Y Moléculas: Bases Complementarias De La Sistemática Moderna, Rafael O. De Sá, David M. Hillis Jan 1993

Morfología Y Moléculas: Bases Complementarias De La Sistemática Moderna, Rafael O. De Sá, David M. Hillis

Biology Faculty Publications

RESUMEN

El auge de las técnicas moleculares en las últimas dos décadas ha traído nuevos enfoques en los estudios sistemáticos. Aquí presentaremos una revisión del uso, las aplicaciones, y los problemas de las principales metodologías de trabajo. Cada una de estas técnicas es aplicable a algunos problemas e inapropiada para otros, y es importante entender las suposiciones y limitaciones de cada técnica. Concluimos que el uso combinado de los enfoques morfológicos y moleculares otorga mejor resolución a los problemas sistemáticos.

ABSTRACT

The development of molecular techniques during the last two decades has brought new approaches to the analysis of systematic …

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