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Full-Text Articles in Molecular Biology
Investigation Of Potentially Catalytic Residues Of Uba5 Through Mutagenesis, Purification, And Structural Characterization, Grant Bradley
Investigation Of Potentially Catalytic Residues Of Uba5 Through Mutagenesis, Purification, And Structural Characterization, Grant Bradley
Senior Honors Projects, 2020-current
Ubiquitin-fold modifier 1 (Ufm1) is a member of the Ubiquitin (Ub) family of proteins whose primary function is degradation of proteins through a sequential mechanism of chemical reactions. Though Ufm1’s specific roles are largely unknown, this family of proteins has shown to play a part in a wide variety of processes, including regulation of the cell cycle1, secretory functions of cells2,3, and blood clotting4. Ufm1’s mechanism of action proceeds with the aid of three enzymes: an E1, E2, and E3. Uba5 is the E1 activating enzyme that is specific to Ufm1, and its mechanism of …
A Proteomic Analysis Of Corydoras Sterbai Secretions And Tissues, Erik Powell Wictor
A Proteomic Analysis Of Corydoras Sterbai Secretions And Tissues, Erik Powell Wictor
University of the Pacific Theses and Dissertations
Defensive mechanisms vary widely in the animal kingdom ranging from physical defenses like spines to chemical defenses such as toxins. Toxins in these secretions and tissues can fluctuate from enzymes to lipids to uncharacterized chemicals. Next generation -omics technology and mass spectrometry are extremely important in analyzing these samples because of their ability to distinguish minute amounts of toxic substance within a complicated sample. The goal of this experiment was to look at secretions and tissues from Corydoras sterbai. All samples in this study were proteolyzed using a mixture of Trypsin and Lys-C, fractionated, and run through nanoLC-MS/MS analysis using …