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Full-Text Articles in Molecular Biology
Regulation Of Β-Casein Gene Expression By Octamer Transcription Factors And Utilization Of Β-Casein Gene Promoter To Produce Recombinant Human Proinsulin In The Transgenic Milk, Xi Qian
Graduate College Dissertations and Theses
β-Casein is a major milk protein, which is synthesized in mammary alveolar secretory epithelial cells (MECs) upon the stimulation of lactogenic hormones, mainly prolactin and glucocorticoids (HP). Previous studies revealed that the proximal promoter (-258 bp to +7 bp) of the β-casein gene is sufficient for induction of the promoter activity by HP. This proximal region contains the binding sites for the signal transducer and activator of transcription 5 (STAT5), glucocorticoid receptor (GR), and octamer transcription factors (Oct). STAT5 and GR are essential downstream mediators of prolactin and glucocorticoid signaling, respectively. This study investigated the functions of Oct-1 and Oct-2 …
Helicase-Ssb Interactions In Recombination-Dependent Dna Repair And Replication, Christian Jordan
Helicase-Ssb Interactions In Recombination-Dependent Dna Repair And Replication, Christian Jordan
Graduate College Dissertations and Theses
Dda, one of three helicases encoded by bacteriophage T4, has been well- characterized biochemically but its biological role remains unclear. It is thought to be involved in origin-dependent replication, recombination-dependent replication, anti- recombination, recombination repair, as well as in replication fork progression past template-bound nucleosomes and RNA polymerase. One of the proteins that most strongly interacts with Dda, Gp32, is the only single-stranded DNA binding protein (SSB) encoded by T4, is essential for DNA replication, recombination, and repair.
Previous studies have shown that Gp32 is essential for Dda stimulation of replication fork progression. Our studies show that interactions between Dda …