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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Biochemical Characterization Of Arsi: A Novel C-As Lyase For Degradation Of Environmental Organoarsenicals, Shashank Suryakant Pawitwar
Biochemical Characterization Of Arsi: A Novel C-As Lyase For Degradation Of Environmental Organoarsenicals, Shashank Suryakant Pawitwar
FIU Electronic Theses and Dissertations
Organoarsenicals such as methylarsenical methylarsenate (MAs(V)) and aromatic arsenicals including roxarsone (4-hydroxy-3-nitrophenylarsenate or Rox(V)) have been extensively used as an herbicide and growth enhancers in animal husbandry, respectively. They undergo environmental degradation to more toxic inorganic arsenite (As(III)) that contaminates crops and drinking water. We previously identified a bacterial gene (arsI) responsible for aerobic MAs(III) demethylation. The gene product, ArsI, is a Fe(II)-dependent extradiol dioxygenase that cleaves the carbon-arsenic (C-As) bond in MAs(III) and trivalent aromatic arsenicals. The objective of this study was to elucidate the ArsI mechanism. Using isothermal titration calorimetry, we determined the dissociation constants (Kd) and ligand-to-protein …