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Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Elcs In Ice: Cryo-Electron Microscopy Of Nuclear Envelope Limited Chromatin Sheets, Mikhail Eltsov, Sergey Sosnovksi, Ada L. Olins, Donald E. Olins
Elcs In Ice: Cryo-Electron Microscopy Of Nuclear Envelope Limited Chromatin Sheets, Mikhail Eltsov, Sergey Sosnovksi, Ada L. Olins, Donald E. Olins
Pharmaceutical Sciences Faculty Posters
Nuclear Envelope-Limited Chromatin Sheets (ELCS) form during excessive interphase nuclear envelope growth in a variety of cells. ELCS appear as extended sheets within the cytoplasm connecting distant nuclear lobes. Cross-section stained images of ELCS, viewed by transmission electron microscopy, resemble a sandwich of apposed nuclear envelopes separated by ~30 nm, containing a layer of ordered chromatin fibers. EM Procedures: The ultrastructure of ELCS was compared by three different methods: 1) aldehyde fixation/dehydration/plastic embedding/sectioning and staining; 2) high-pressure freezing/freeze substitution into plastic/sectioning and staining; 3) high-pressure freezing/cryo-sectioning/cryo-electron microscopy. Human leukemic (HL-60/S4) cells were treated with retinoic acid (4 days) to induce …
Cloning And Biochemical Characterization Of The Recq Helicase And Topoisomerase Iii In Schizosaccharomyces Pombe, Rebecca Wood
Cloning And Biochemical Characterization Of The Recq Helicase And Topoisomerase Iii In Schizosaccharomyces Pombe, Rebecca Wood
All Theses And Dissertations
RecQ and Topoisomerase III proteins are both essential for proper chromosomal maintenance in species ranging from bacteria to humans. Due to their highly conserved nature throughout evolution, understanding their basic biochemical properties as well as their interactions together is important for understanding human health, disease, and even to provide potential evolutionary insight. S. pombe has been chosen as a model organism because little research has been done on the biochemical analysis of its RecQ homolog, Rqhl. This study shows developments in the biochemical characterization of a truncation protein of Rqh1 including AFM (Atomic Force Microscopy) Imaging, DNA binding, and ATP …