Biochemistry, Biophysics, and Structural Biology Commons^™

Assay Replicability In Β-Glucosidase Enzyme Kinetics Across Laboratories, Nicole-Tia Najam, Jaime Mayoral

FIU Undergraduate Research Journal

Replicability is the foundation of research in any scientific discipline. Despite this fact, few studies address experimental variability within and across multiple institutions that operate under the same protocol. While consistency is usually well documented within the same lab, multi-institutional experiments may introduce new variables and, therefore, variability that may lead to inconsistent results. This study seeks to explore intra- and interinstitutional variability among enzyme catalytic efficiency values (K_M and K_cat/K_M) for the wild type of β-Glucosidase derived from Paenibacillus polymyxa. A standardized protocol for the assay was provided to all institutions that participated in …