Open Access. Powered by Scholars. Published by Universities.®
Biochemistry, Biophysics, and Structural Biology Commons™
Open Access. Powered by Scholars. Published by Universities.®
- Discipline
- Keyword
-
- Apoptosis (1)
- Beta-glucosidase (1)
- Cancer (1)
- Coevolution (1)
- DNA (1)
-
- DNA Damage (1)
- Evolutionary genetics (1)
- Inhibitors (1)
- Inhibitory Activity (1)
- Inter-laboratory (1)
- Intra-laboratory (1)
- Kinetics (1)
- Melatonin (1)
- Multicellular (1)
- Neurotransmitter (1)
- Oncogene (1)
- Oncogenesis (1)
- P53 gene family (1)
- Protein purification (1)
- Recombinant protein (1)
- Replicability (1)
- Selective Binding (1)
- TP53 (1)
- TP63 (1)
- TP73 (1)
- Topoisomerase III (1)
- Tumor supressing genes (1)
- Type IA Topoisomerase (1)
- Wild-type (1)
Articles 1 - 4 of 4
Full-Text Articles in Biochemistry, Biophysics, and Structural Biology
Purification Of Recombinant E. Coli Topoisomerase Iii For Structure-Based Drug Design Using Protein Crystallization, Miguel A. Perez Rodriguez, Yuk-Ching Tse-Dinh
Purification Of Recombinant E. Coli Topoisomerase Iii For Structure-Based Drug Design Using Protein Crystallization, Miguel A. Perez Rodriguez, Yuk-Ching Tse-Dinh
FIU Undergraduate Research Journal
Type IA Topoisomerases are ubiquitous enzymes found throughout all life forms and species. These topoisomerases relieve the topographical constrains formed by DNA during processes like replication and transcription via a cleavage-religation mechanism performed through a catalytically active tyrosine residue in the primary structure of the enzyme. E. coli Topoisomerase III (EtopIII) is a type of Type IA topoisomerase, and its main function in the cell is as a decatenase, which means that it unlinks circular or intertwined pieces of genetic material and creates two unlinked segments of DNA from a singular linked chain. Structure-based determination of the enzyme’s three-dimensional structure …
The Evolution Of Tumor Suppressing Genes In Multicellular Organisms: Nature’S Prevention Of Oncogenesis, Melanie Perez
The Evolution Of Tumor Suppressing Genes In Multicellular Organisms: Nature’S Prevention Of Oncogenesis, Melanie Perez
FIU Undergraduate Research Journal
The p53 gene family, a well-known group of genes, is the primary propagator of tumor-suppressing mechanisms in multicellular organisms. Although they are currently critical drug targets in cancer, the p53 family also serves specific functions in the development of multicellular organisms. In this paper, the current function, origin, and evolutionary purpose of the p53 family are reviewed in the evolution of multicellular organisms. The TP53 gene induces cellular responses such as apoptosis as a way to combat detrimental environmental and cellular factors that can damage the integrity of a cell’s DNA. The other two members of the p53 family are …
Assay Replicability In Β-Glucosidase Enzyme Kinetics Across Laboratories, Nicole-Tia Najam, Jaime Mayoral
Assay Replicability In Β-Glucosidase Enzyme Kinetics Across Laboratories, Nicole-Tia Najam, Jaime Mayoral
FIU Undergraduate Research Journal
Replicability is the foundation of research in any scientific discipline. Despite this fact, few studies address experimental variability within and across multiple institutions that operate under the same protocol. While consistency is usually well documented within the same lab, multi-institutional experiments may introduce new variables and, therefore, variability that may lead to inconsistent results. This study seeks to explore intra- and interinstitutional variability among enzyme catalytic efficiency values (KM and Kcat/KM) for the wild type of β-Glucosidase derived from Paenibacillus polymyxa. A standardized protocol for the assay was provided to all institutions that participated in …
Sequence Selectivity In The Binding Of Melatonin To Phix174rf Dna, Camila Garcia
Sequence Selectivity In The Binding Of Melatonin To Phix174rf Dna, Camila Garcia
FIU Undergraduate Research Journal
Melatonin is an endogenous neurotransmitter that controls the circadian rhythm. When consumed through medication, it has been proven to serve as a treatment for sleep disorders and alleviate sleep quality. Stable levels of melatonin in the human organism are shown to be beneficial as it provides a protective layer to DNA strands ensuring their longevity; however, its excessive consumption may have damaging consequences on the long run. Here we present a study on the sequence selectivity in the binding of melatonin to DNA, intending to show that melatonin targets specific DNA sites. Binding tophiX174RF DNA was assayed using restriction enzyme …