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Cdna Sequences Of The Caprine Gamma Delta T Cell Hybrid Co-Receptor And Pathogen Recognition Receptor Wc1 Multigene Family, Maria Solangi

Masters Theses

Workshop cluster 1 (WC1) molecules are exclusively expressed on the surface of gamma delta T cells and act as co-receptors and bind pathogens thus also functioning as pattern recognition receptors. The aim was to obtain cDNA evidence to support the recent caprine genome annotation of the WC1 multigene family conducted by a colleague. To get cDNA sequences three strategies were used. Strategy 'I' was used to obtain three clones that corresponded to WC1 SRCR domain d9 through the intracytoplasmic tail sequence. Strategy 'II' was used to obtain 6 clones. A PCR was conducted using SRCR domain b7 through the intracytoplasmic …