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Full-Text Articles in Other Animal Sciences

Effects Of Maternal Infant-Directed Sensory Cues On Early Weaned Piglet Behavior, Mattie Still May 2024

Effects Of Maternal Infant-Directed Sensory Cues On Early Weaned Piglet Behavior, Mattie Still

Animal Science Undergraduate Honors Theses

In the commercial pig production industry, weaning is a stressful time. Stress is related to loss in production due to increased incidence of illness, increased aggression, and reduced rate of gain, which results in economic losses for the producer. The purpose of this continuous behavior study was to explore the effects of provisional own-mother olfactory and auditory stimuli on early weaned piglet behavior and growth. During days 7-14 postpartum, the grunting of 12 individual sows was recorded while their piglets nursed. The audio files were put into a single loop with grunting occurring for 20 minutes followed by 60 minutes …


Exploring A Potential Bias In Detection Of Mesopredators By Cameras, Rylee Gibson May 2023

Exploring A Potential Bias In Detection Of Mesopredators By Cameras, Rylee Gibson

Biological Sciences Undergraduate Honors Theses

Mesopredators, such as the raccoon (Procyon locor), Virginia opossum (Didpelphis virginiana), and striped skunk (Mephitis mephitis) play crucial ecological roles as predators, prey, and disease vectors across much of the United States. Because of their importance and the way that populations of these mesopredators can dramatically increase due to human-subsidized resources, it is imperative that studies attempting to quantify mesopredator community composition are accurate and unbiased. However, it has recently been suggested that not all mammals trigger motion-activated wildlife game cameras at the same rate and for some species detection probability may be biased. My goals for this thesis were …


Pilot Testing A Survey To Evaluate Horse Owner And Trainer Perceptions Of The Importance And Ideality Of Equine Hoof Balance, Katherine Gilmore Dec 2022

Pilot Testing A Survey To Evaluate Horse Owner And Trainer Perceptions Of The Importance And Ideality Of Equine Hoof Balance, Katherine Gilmore

Animal Science Undergraduate Honors Theses

Balance plays an integral role in the healthy equine hoof, yet there is a significant deficiency of research investigating horse owner and trainer knowledge of hoof balance. This study aimed to pilot test an online survey instrument evaluating horse owner and trainer perceptions of proper hoof care and balance. The survey instrument sought to collect demographic information of horse owners and trainers, determine horse owners’ and trainers’ abilities to identify ideally balanced equine forehooves, and assess horse owner and trainer knowledge of best hoof trimming and shoeing practices. Ninety-one horse owners and trainers with facilities that were within a 150-mile …


Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. Desmet May 2022

Pandemics And Animal Welfare: A Quantitative Inquiry Into How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Kayla N. Desmet

Animal Science Undergraduate Honors Theses

Since the emergence of the COVID-19 pandemic in late 2019, industries have been impacted globally. The companion animal care industry has not received much recognition in mainstream news, and though multiple grants have been allocated (Nolen, 2020), limited resources were available to individual facilities such as animal shelters, animal day cares, and boarding facilities. Little is known about the long-term effects of the pandemic on these facilities and how companion animal care may have changed. The purpose of this study was to explore the day-to-day impacts and long-term effects of COVID-19 on the companion animal industry in Northwest Arkansas from …


Pandemics And Animal Welfare: How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Sabrina Cox May 2022

Pandemics And Animal Welfare: How The Covid-19 Pandemic Has Affected The Companion Animal Industry In Northwest Arkansas, Sabrina Cox

Animal Science Undergraduate Honors Theses

The first cases of coronavirus reported in the United States came in January 2020 (World Health Organization, 2020). In the two years since then, countries around the world have been affected by COVID-19 and the subsequent social, economic, and mental health issues. Previous research shows that the animal healthcare field is already overrun with burnout, substance abuse, depression, and increased levels of stress (Gardner & Hini, 2006), and the pandemic has only compounded these issues (Mair et al., 2020). Observing a lack of meaningful reporting on the issues affecting veterinary staff led the researchers to investigate staff members’ practices, day- …


Impact Of A Cattle Crossing On Water Quality Along A Tributary Of The Muddy Fork Of The Illinois River, Northwest Arkansas, Nicholas Valentini May 2022

Impact Of A Cattle Crossing On Water Quality Along A Tributary Of The Muddy Fork Of The Illinois River, Northwest Arkansas, Nicholas Valentini

Animal Science Undergraduate Honors Theses

Cattle are known to have an effect on water quality in various bodies of water. Studying how cattle impact water quality along various streams and tributaries is important to understanding how certain water parameters may be affected at the individual farm level. It is known that unrestricted access to a cattle crossing has been shown to increase the occurrence of downstream pollutants such as E. coli, ammonium, total kjeldahl nitrogen, total suspended solids, total phosphorus, and turbidity. However, many studies focus on large-scale operations and neglect the individual farm level. In this study, samples were collected twice for each …


Effects Of Cottonseed Meal Containing Gossypol On Testis Physiology In Boars, Kristin Ryan May 2022

Effects Of Cottonseed Meal Containing Gossypol On Testis Physiology In Boars, Kristin Ryan

Animal Science Undergraduate Honors Theses

Feral hogs are an invasive species found in 35 U.S. states without an effective control method to limit their population growth. According to a 2019 survey of 467 landowners in Arkansas, feral hogs caused an estimated $12 million in damages (Cook, 2019). While there are current control methods such as hunting and trapping, 65% of the feral hog population must be eliminated in a specific area to prohibit population growth (Cook, 2019). It is theorized that gossypol, a phenolic compound known to negatively impact reproductive function in other species, could cause sterility in boars. In this preliminary research study, 21 …


Sublethal Effects Of Red Imported Fire Ant Envenomation On Hatchlings Of North American Oviparous Snakes, Hannah Warner, Meredith Swartwout May 2021

Sublethal Effects Of Red Imported Fire Ant Envenomation On Hatchlings Of North American Oviparous Snakes, Hannah Warner, Meredith Swartwout

Crop, Soil and Environmental Sciences Undergraduate Honors Theses

Invasive species cause major ecological and economic damage. The Red Imported Fire Ant (RIFA; Solenopsis invicta) has successfully invaded much of the southeastern United States and has caused both widespread economic damage and is suspected to be the driver of enigmatic declines of several oviparous snake species. This study aimed to determine the sublethal effects of RIFA on hatchlings of six species of oviparous snakes (Coluber constrictor, Lampropeltis calligaster, L. holbrooki, Opheodrys aestivus, Pantherophis emoryi, and P. obsoletus) by exposing hatchlings to envenomation by RIFA and then measuring two performance metrics: righting response and …


Effects Of Temporal Variation On Ambient Light In Northwest Arkansas, Ashlyn Anderson Dec 2020

Effects Of Temporal Variation On Ambient Light In Northwest Arkansas, Ashlyn Anderson

Biological Sciences Undergraduate Honors Theses

An animal’s life success is determined solely by its fitness, which makes choosing a mate one of its most important life decisions. Natural selection plays a big part in an animal’s phenotype, but so does sexual selection. Even though females are usually thought to be the choosier sex, in many species or seasons males are also choosy. Male mate preference is an understudied topic compared to female mate preference and therefore, even less is known about the outcomes of a male’s prior mating experience’s influence on future mating experiences. Therefore, I dove deeper into this topic with the highly studied …


Pilot Testing A Qualitative Methodology To Evaluate The Perceived Benefits And Stressors Of Sole-Dog Ownership In Full-Time College Students, Emma Williams May 2020

Pilot Testing A Qualitative Methodology To Evaluate The Perceived Benefits And Stressors Of Sole-Dog Ownership In Full-Time College Students, Emma Williams

Animal Science Undergraduate Honors Theses

The number of individuals acquiring pets is continually increasing, with 71.5% of Americans owning a pet in 2012. Companion animals are a huge source of benefits but owning them can also come with several stressors. However, little research has been conducted on the negative aspects of dog ownership. This pilot test evaluates the perceived stressors and benefits of sole dog ownership in full-time college students. The pilot aimed to hold three focus groups from which qualitative thematic data were collected and quantitative were collected from a demographic survey of participants. Participants were asked a series of questions about sole dog …


Use Of Diatomaceous Earth And Copper Oxide Wire Particles To Control Gastrointestinal Nematodes In Lambs, Olivia Jones May 2020

Use Of Diatomaceous Earth And Copper Oxide Wire Particles To Control Gastrointestinal Nematodes In Lambs, Olivia Jones

Animal Science Undergraduate Honors Theses

Abstract

Anthelmintic resistance (AR) urges alternatives to control gastrointestinal nematodes (GIN). Copper oxide wire particles (COWP) are more efficacious when used with other dewormers and little is known on efficacy of diatomaceous earth (DE) to control gastrointestinal parasites. The objective was to examine the effects of DE and COWP on GIN control. Katahdin lambs (n = 32; ~150 d of age; 25.0 ± 1.8 kg) were randomly assigned to receive: 1) DE fed at an estimated 2% dry matter intake (with the assumption of moderate consumption of bermudagrass forage and provided supplement), 2) 1g COWP, 3) both 2% DE and …


Differences In Learning And Gene Expression In Brains Of Male And Female Bicyclus Anynana, Gabrielle Agcaoili May 2020

Differences In Learning And Gene Expression In Brains Of Male And Female Bicyclus Anynana, Gabrielle Agcaoili

Biological Sciences Undergraduate Honors Theses

One way to understand the variation in the behavior of animals is by looking at the genes involved. We were particularly interested in behavioral differences between the sexes. How might these differences be manifested in the brain? This study worked to answer this question by using male and female butterflies of the species Bicyclus anynana, examining what is going on in the brains of males and females as they learned from a social exposure. We focused in on how sex plays a role in behavior and learning; it has been seen that males and females respond to the same social …


The Effects Of Pre And Post Exercise Low-Level Laser Therapy On Biochemical Markers Of Skeletal Muscle Fatigue In Equines, Carson Freeman May 2019

The Effects Of Pre And Post Exercise Low-Level Laser Therapy On Biochemical Markers Of Skeletal Muscle Fatigue In Equines, Carson Freeman

Animal Science Undergraduate Honors Theses

Our objective was to determine whether administering low-level laser therapy before or after exercise had the greatest effect on biochemical markers of skeletal muscle fatigue in equines such as cortisol and blood lactate. Twelve quarter horses were divided into three groups: Group A received no laser therapy, Group B received laser therapy before exercise, and Group C received laser therapy after exercise. A Class II ERCHONIA ® PL500 handheld low-level laser was utilized for treatment with a wavelength of 635nm. Exercise was utilized using a horse walker system for 30 minutes five days a week for three weeks. Blood was …


The Diversity Of Terrestrial Mammals Surrounding Waterfall At Billy Barquedier National Park, Kelsey Johnson May 2019

The Diversity Of Terrestrial Mammals Surrounding Waterfall At Billy Barquedier National Park, Kelsey Johnson

Animal Science Undergraduate Honors Theses

Billy Barquedier is a National Park located in the Stann Creek district of Belize that contains Neotropical vegetation and wildlife. This study was performed in order to provide a baseline inventory and appearance frequency patterns of the terrestrial mammals located within Zone 1 of the park near a waterfall. The goal of the data and information collected during this study is to gain a greater understanding of the biodiversity and activity patterns of terrestrial mammals within the park and across Belize as a whole. The use of camera traps, small Sherman live traps, large live traps, and tracking methods were …


Relationships Among Beef Cow Productivity Traits And Single Nucleotide Polymorphisms In The Bovine Heat Shock Protein 70 Gene, Ashley Finney May 2018

Relationships Among Beef Cow Productivity Traits And Single Nucleotide Polymorphisms In The Bovine Heat Shock Protein 70 Gene, Ashley Finney

Animal Science Undergraduate Honors Theses

When eukaryotes are exposed to stressors such as heat, toxins, and low oxygen levels, heat shock proteins (HSPs) are synthesized to maintain normal cellular function within the body. Single nucleotide polymorphisms in the heat shock protein 70 (Hsp70) gene have been associated with calving percentage, and Julian calving date in spring-calving crossbred Brahman cows (Rosenkrans, et al., 2010). Our objective was to determine associations between previously identified polymorphisms in the promoter region and coding sequence of the bovine Hsp70 gene and beef cow efficiency. We evaluated productivity traits, including Julian calving date and calving rates, of fall-calving cows at each …


Polymorphisms Of Bovine Hsp90 And Their Implications In Beef Cattle Productivity, Glynn G. Smith May 2018

Polymorphisms Of Bovine Hsp90 And Their Implications In Beef Cattle Productivity, Glynn G. Smith

Animal Science Undergraduate Honors Theses

Production of beef cattle represents a $60 billion industry in the United States (USDA, 2015). The American beef cattle industry loses an estimated $370 million annually due to heat stress (St-Pierre, 2003). As of 2003, this was equal to nearly 99 million pounds of beef lost (USDA, 2015). The average American consumed roughly 65 pounds of beef in 2003; this means that the 99 million pounds of beef lost to heat stress would have been enough to feed approximately 1.5 million Americans for an entire year (Barclay, 2012).


Impact Of Teat Order On Feed Consumption In Swine From Birth To Nursery, Callan Lichtenwalter May 2018

Impact Of Teat Order On Feed Consumption In Swine From Birth To Nursery, Callan Lichtenwalter

Animal Science Undergraduate Honors Theses

A relationship between teat order and feed consumption has been assumed in pigs, but no study has looked at this exact relationship. Pigs were observed shortly after birth to be in either a cranial, middle, or caudal teat positon. Growth performance data and active and total plasma ghrelin concentrations were analyzed at birth, weaning, and at the end of the nursery stage of production to see if a relationship with teat order was present. Further growth performance data were analyzed during different phases of the nursery stage. Overall, no effect of teat order was found on average daily gain, average …


The Effects Of Super-Dosing Phytase On Nursery And Grower Pigs, Joshua Paul Knapp Jan 2018

The Effects Of Super-Dosing Phytase On Nursery And Grower Pigs, Joshua Paul Knapp

Graduate Theses and Dissertations

To determine the effects of super-dosing on pigs fed phosphorous and calcium adequate or deficient diets (Experiment 1); the optimum level of corn express phytase (CEP, Experiment 2); and the effects of xylanase/phytase on growth performance (Experiment 3) in pigs fed reduce nutrient diets, weaned pigs were blocked with initial body weight (BW) and allotted to dietary treatments. Treatments were: Positive control (PC), P/Ca adequate diets (NRC 2012); Negative Control (NC), low P (-0.15%) and Ca (-0.12%), +0, 1000, and 4000 FTU/kg of CEP in both PC and NC (Experiment 1); PC, P/Ca adequate diets; NC, decreased P (0.15%) and …


The Effect Of Pet Ownership On Esl Students’ U.S. Academic Success, Emily Benjumea Dec 2017

The Effect Of Pet Ownership On Esl Students’ U.S. Academic Success, Emily Benjumea

Animal Science Undergraduate Honors Theses

This study examines the academic success of English as a second language (ESL) students based on ownership of and/or relationship with a pet, due to pets’ ability to benefit and support their owners. A survey was compiled for middle school, ESL students in the Fayetteville, Arkansas school district. The goal of the study was to discover if pet ownership has an effect on ESL student academic success in terms of the academic, social, and emotional aspects of school. It was found that pets aid in some academic aspects of school but more so in the social and emotional aspects such …


Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleukin-8 Receptor Genes Are Related To Production Traits And Hair Coat Score In Crossbred Cattle, Avery Deaton May 2017

Genetic Polymorphisms Of The Glucocorticoid Receptor And Interleukin-8 Receptor Genes Are Related To Production Traits And Hair Coat Score In Crossbred Cattle, Avery Deaton

Animal Science Undergraduate Honors Theses

The objective of this thesis was to identify polymorphisms in the glucocorticoid receptor (GR) and interleukin-8 receptor (CXCR2) genes and to associate genotypes between the above mentioned polymorphisms and production traits in crossbred cattle. The hypothesis was that polymorphisms will exist for GR and CXCR2 genes and will be linked to production traits. Glucocorticoid receptors have been positively associated with higher milk yields, lactose content, feed intake, and feed conversion rates. Interleukin-8 genes are part of the innate immune response and help with many aspects of female reproduction health, such as protecting the embryo from the maternal immune system during …


Use Of Anti-Mullerian Hormone To Select For Fertility In Beef Heifers, Hannah Newberry May 2016

Use Of Anti-Mullerian Hormone To Select For Fertility In Beef Heifers, Hannah Newberry

Animal Science Undergraduate Honors Theses

ABSTRACT

A study was conducted to determine whether concentration of serum Anti-Mullerian Hormone (AMH) at weaning and/or breeding could predict subsequent fertility in beef heifers. Frequency distribution was used to assign serum AMH concentration measured at weaning, breeding, and the change from weaning to breeding, into quartiles. Comparison of heifers based on serum AMH quartiles at weaning failed (P ³ 0.35) to detect any effect of AMH on subsequent heifer cyclicity at breeding, estrous response after synchronization, artificial insemination (AI) pregnancy rate, overall breeding season pregnancy rate, or estimated estrous cycle of the breeding season when conception occurred. Based on …


Broiler Breeder Mating Behavior And The Effects Of Pre-Incubation Embryo Integrity Modifications On Broiler Performance, Emily Kathryn Lhamon Dec 2015

Broiler Breeder Mating Behavior And The Effects Of Pre-Incubation Embryo Integrity Modifications On Broiler Performance, Emily Kathryn Lhamon

Graduate Theses and Dissertations

Modern broiler breeders have been observed displaying significantly less mating behaviors than their ancestral fowl. Effective mating behavior translates into fertile hatching eggs that ultimately become broilers processed for market. The poultry industry has made significant changes in genetics, environment, housing, and diets of commercial poultry. However, there are some concessions made in genetic selection and streamlined management to produce larger, fast-growing, efficient birds. Aggression in broiler breeder males is believed to be a sign of high fertility. However, highly aggressive males pose a threat to the welfare of their flock mates, and may also impede frequency of mating and …


The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark Dec 2015

The Effect Of Alternative Fertilization And Application Time On Voluntary Intake Of Forages, Jessica Clark

Graduate Theses and Dissertations

Dairy slurry and poultry litter have increased in popularity as fertilizers in the agriculture industry. However, residual effects of these manures on voluntary intake of forages from subsequent harvests are not well known. The objectives of this two part study were to determine if moisture level of alfalfa silage and the use of dairy slurry as a fertilizer have an effect on intake and digestibility by sheep, and if forage species and the use of poultry litter as a fertilizer have an effect on intake by horses. Eighteen ewes (47.6 ± 5.34 kg) were used in experiment 1 and were …


Effect Of Feeding Regimen And Age On Lipid Metabolism In Broiler Breeder Hens And Progeny, Nirun Boonsinchai Jul 2015

Effect Of Feeding Regimen And Age On Lipid Metabolism In Broiler Breeder Hens And Progeny, Nirun Boonsinchai

Graduate Theses and Dissertations

Age of hens and feed allocation can affect egg yolk lipid compositions which in turn directly relate to chick quality. A series of experiments were conducted to determine the effects of age and feeding programs on lipid metabolism in breeder hens and progeny. In the first trial, the rate of yolk absorption and fatty acid profiles in the yolk were compared among 28, 35, and 49wk old hens. The results revealed that the rate of yolk absorption was higher, but the absolute amount of fatty acids absorbed was lower in young hens than in the old hens. The second trial …


Life Cycle Assessment Of Alternative Swine Management Practices, Prathamesh Avadhut Bandekar Jul 2015

Life Cycle Assessment Of Alternative Swine Management Practices, Prathamesh Avadhut Bandekar

Graduate Theses and Dissertations

Life Cycle Assessments (LCAs) are quantitative analyses of complex systems for evaluation of impacts and risk associated with management decisions. LCAs can be effective tools for determining comparative advantages of management strategies across specific impact concern. In this study, life cycle assessments of pork production management alternatives was performed. The alternative management practices included in this study were production of entire males (boars), use of pens for gestation housing, immunocastration, production without growth promoting antimicrobials, production without growth promoting and preventive antimicrobials, and production without ractopamine. These LCAs evaluated the impact of each management strategy on greenhouse gas emission (GHG), …


A Cradle To Farm Gate Life Cycle Analysis Of Land Use In U.S. Pork Production, William Benjamin Putman Jul 2015

A Cradle To Farm Gate Life Cycle Analysis Of Land Use In U.S. Pork Production, William Benjamin Putman

Graduate Theses and Dissertations

The goal of this study was to conduct a detailed Life Cycle Assessment (LCA) of the U.S. live swine production supply chain to quantify land use requirements and to assess the impact associated with various ration compositions. The functional unit was defined as one kilogram (2.2 pounds) of live swine at the farm gate, ready for transport to the abattoir. This assessment focused on the three highest producing USDA regions, which encompassed the Midwest (Regions 5 and 7) and the Southeast (Region 4), representing 86% of U.S. market hog production.

First, a literature review was conducted to summarize the most …


Use Of Endocrine And Immune Responses As Predictors Of Bull Sperm Motility, Lydia Mitchener May 2015

Use Of Endocrine And Immune Responses As Predictors Of Bull Sperm Motility, Lydia Mitchener

Animal Science Undergraduate Honors Theses

Research has shown that immune cells increased from an immune response, and endocrine concentrations directly affect sperm characteristics (Jones and Mann, 1976; Hansson et al., 1989; Grattan et al., 2007). Previous findings show a negative relationship between leukocytes and sperm function (Jones and Mann, 1976) and prolactin and fertility (Grattan et al., 2007). On the other hand, research has shown a positive relationship between insulin-like growth factor (IGF) and sperm characteristics (Hansson et al., 1989). The objective of this study is to identify biomarkers for yearling bull sperm associated with endocrine response and activation of the immune system. Seventeen Brahman-influenced …


Effects Of Crystalline Amino Acid Supplementation Of Reduced Crude Protein (Rcp) Diet On Net Energy Basis On Growing-Finishing Swine, Daniel Cook May 2015

Effects Of Crystalline Amino Acid Supplementation Of Reduced Crude Protein (Rcp) Diet On Net Energy Basis On Growing-Finishing Swine, Daniel Cook

Graduate Theses and Dissertations

Barrows and gilts (n=210) were used to test the effects of crystalline amino acid (AA) supplementation of reduced crude protein (RCP) diets formulated on a net energy basis on quality characteristics of the LM from growing-finishing pigs. Pigs were blocked by weight, and pens (3 barrows and 3 gilts/pen) within each block were assigned randomly to either corn-soybean meal diets (C) or 1 of 3 RCP diets and added crystalline AA levels for the dietary treatments during each feeding phase. During the last 3-wk feeding phase, 10 ppm of ractopamine were included in all diets. At slaughter and after a …


Assessing The Effectiveness Of Student Oriented Learning Outlines (Solos) In An Equine Classroom, Kathleen S. Jogan Aug 2014

Assessing The Effectiveness Of Student Oriented Learning Outlines (Solos) In An Equine Classroom, Kathleen S. Jogan

Graduate Theses and Dissertations

This study determined if the use of the student oriented learning outline (SOLO) in a University of Arkansas equine production classroom had a positive influence in three areas: mastery of material taught, retention of material taught and voluntary positive student behaviors related to the use of course material. Thirty-one students who were enrolled in 2012 were in the non-SOLO group (control), and 25 students who were enrolled in 2013 were the SOLO group (treatment). Three separate units were taught to the treatment and control groups with only one difference: the treatment group was given a SOLO one week in advance …


Effect Of Supplemental Trace Mineral (Zinc, Copper, And Manganese) Source On Growth Performance, Morbidity, And Trace Mineral Status In Beef Cattle, Anthony Ryan Aug 2014

Effect Of Supplemental Trace Mineral (Zinc, Copper, And Manganese) Source On Growth Performance, Morbidity, And Trace Mineral Status In Beef Cattle, Anthony Ryan

Graduate Theses and Dissertations

A series of studies were conducted to determine the effect of supplemental trace mineral source on growth performance, morbidity, and trace mineral status in growing beef cattle. The first experiment evaluated supplemental trace minerals from sulfate, organic, or hydroxy sources on growth performance and morbidity. Crossbreed beef calves were assigned to 1 of 3 treatments consisting of supplemental zinc (360 mg/d), copper (125 mg/d), and manganese (200 mg/d) from inorganic, organic, or hydroxy sources fed daily over a 42 to 45-d backgrounding phase. After removal of chronic and dead calves from the data set, trace mineral source had no effect …