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Full-Text Articles in Meat Science
Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel
Genes Involved In Feed Efficiency Identified In A Meta-Analysis Of Rumen Tissue From Two Populations Of Beef Steers, Amanda K. Lindholm-Perry, Allison M. Meyer, Rebecca J. Kern-Lunbery, Hannah C. Cunningham-Hollinger, Taran H. Funk, Brittney N. Keel
Roman L. Hruska U.S. Meat Animal Research Center: Reports
In cattle, the rumen is an important site for the absorption of feed by-products released by bacterial fermentation, and variation in ruminal function plays a role in cattle feed efficiency. Studies evaluating gene expression in the rumen tissue have been performed prior to this. However, validating the expression of genes identified in additional cattle populations has been challenging. The purpose of this study was to perform a meta-analysis of the ruminal transcriptome of two unrelated populations of animals to identify genes that are involved in feed efficiency across populations. RNAseq data from animals with high and low residual feed intake …
Untangling The Placentome Gene Network Of Beef Heifers In Early Gestation, Wellison J.S. Diniz, Lawrence P. Reynolds, Alison K. Ward, Pawel P. Borowicz, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen
Untangling The Placentome Gene Network Of Beef Heifers In Early Gestation, Wellison J.S. Diniz, Lawrence P. Reynolds, Alison K. Ward, Pawel P. Borowicz, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen
Department of Animal Science: Faculty Publications
The cotyledon and caruncle tissues provide a functional bridge between the fetus and the dam. However, the relationship between these tissues and the transcriptomic profile that underlies the tissue functions remains elusive. Herein we investigate the expression profile of cotyledon and caruncle from nulliparous beef heifers carrying female fetuses at day 83 of pregnancy to identify changes occurring across tissues that contribute to placental function and their tissue-specific roles. We identified 2654 differentially expressed genes [padj ≤ 0.05, abs(log2FC) ≥ 1], including nutrient transporters and paternally imprinted genes. We found key regulators of tissue function and differentiation, including FOXO4, GATA2, …
Transcriptome Profiles Of The Skeletal Muscle Of Mature Cows During Feed Restriction And Realimentation, Hannah C. Cunningham-Hollinger, Larry A. Kuehn, Kristi M. Cammack, Kristin E. Hales, William T. Oliver, Matthew S. Crouse, Celine Chen, Harvey C. Freetly, Amanda K. Lindholm-Perry
Transcriptome Profiles Of The Skeletal Muscle Of Mature Cows During Feed Restriction And Realimentation, Hannah C. Cunningham-Hollinger, Larry A. Kuehn, Kristi M. Cammack, Kristin E. Hales, William T. Oliver, Matthew S. Crouse, Celine Chen, Harvey C. Freetly, Amanda K. Lindholm-Perry
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Objective: Realimentation can compensate for weight loss from poor-quality feedstuffs or drought. Mature cows fluctuate in body weight throughout the year due to nutrient availability. The objective of this study was to determine whether cows that differ in weight gain during realimentation also differ in the abundance of transcripts for enzymes associated with energy utilization in skeletal muscle. Mature cows were subjected to feed restriction followed by ad libitum feed. Skeletal muscle transcriptome expression differences during the two feeding periods were determined from cows with greater (n = 6) and less (n = 6) weight gain during the ad libitum …
Decoding The Equine Genome: Lessons From Encode, Sichong Peng, Jessica L. Petersen, Rebecca R. Bellone, Ted Kalbfleisch, N. B. Kingsley, Alexa Barber, Eleonora Cappelletti, Elena Giulotto, Carrie J. Finno
Decoding The Equine Genome: Lessons From Encode, Sichong Peng, Jessica L. Petersen, Rebecca R. Bellone, Ted Kalbfleisch, N. B. Kingsley, Alexa Barber, Eleonora Cappelletti, Elena Giulotto, Carrie J. Finno
Department of Animal Science: Faculty Publications
The horse reference genome assemblies, EquCab2.0 and EquCab3.0, have enabled great advancements in the equine genomics field, from tools to novel discoveries. However, significant gaps of knowledge regarding genome function remain, hindering the study of complex traits in horses. In an effort to address these gaps and with inspiration from the Encyclopedia of DNA Elements (ENCODE) project, the equine Functional Annotation of Animal Genome (FAANG) initiative was proposed to bridge the gap between genome and gene expression, providing further insights into functional regulation within the horse genome. Three years after launching the initiative, the equine FAANG group has generated data …
Reference Transcriptomes Of Porcine Peripheral Immune Cells Created Through Bulk And Single-Cell Rna Sequencing, Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher Tuggle
Reference Transcriptomes Of Porcine Peripheral Immune Cells Created Through Bulk And Single-Cell Rna Sequencing, Juber Herrera-Uribe, Jayne E. Wiarda, Sathesh K. Sivasankaran, Lance Daharsh, Haibo Liu, Kristen A. Byrne, Timothy P. L. Smith, Joan K. Lunney, Crystal L. Loving, Christopher Tuggle
Roman L. Hruska U.S. Meat Animal Research Center: Reports
Pigs are a valuable human biomedical model and an important protein source supporting global food security. The transcriptomes of peripheral blood immune cells in pigs were defined at the bulk cell-type and single cell levels. First, eight cell types were isolated in bulk from peripheral blood mononuclear cells (PBMCs) by cell sorting, representing Myeloid, NK cells and specific populations of T and B-cells. Transcriptomes for each bulk population of cells were generated by RNA-seq with 10,974 expressed genes detected. Pairwise comparisons between cell types revealed specific expression, while enrichment analysis identified 1,885 to 3,591 significantly enriched genes across all 8 …
A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee Mcfee Fee, Sarah Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp
A High-Androgen Microenvironment Inhibits Granulosa Cell Proliferation And Alters Cell Identity, Renee Mcfee Fee, Sarah Romereim, Alexandria P. Snider, Adam F. Summers, William E. Pohlmeier, Scott G. Kurz, Robert A. Cushman, John S. Davis, Jennifer R. Wood, Andrea S. Cupp
Department of Animal Science: Faculty Publications
A naturally occurring bovine model with excess follicular fluid androstenedione (High A4), reduced fertility, and polycystic ovary syndrome (PCOS)-like characteristics has been identified. We hypothesized High A4 granulosa cells (GCs) would exhibit altered cell proliferation and/or steroidogenesis. Microarrays of Control and High A4 GCs combined with Ingenuity Pathway Analysis indicated that High A4 GCs had cell cycle inhibition and increased expression of microRNAs that inhibit cell cycle genes. Granulosa cell culture confirmed that A4 treatment decreased GC proliferation, increased anti-Müllerian hormone, and increased mRNA for CTNNBIP1. Increased CTNNBIP1 prevents CTNNB1 from interacting with members of the WNT signaling pathway thereby …
Maternal Vitamin And Mineral Supplementation And Rate Of Maternal Weight Gain Affects Placental Expression Of Energy Metabolism And Transport-Related Genes, Wellison J. S. Diniz, Lawrence P. Reynolds, Pawel P. Borowicz, Alison K. Ward, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen
Maternal Vitamin And Mineral Supplementation And Rate Of Maternal Weight Gain Affects Placental Expression Of Energy Metabolism And Transport-Related Genes, Wellison J. S. Diniz, Lawrence P. Reynolds, Pawel P. Borowicz, Alison K. Ward, Kevin K. Sedivec, Kacie L. Mccarthy, Cierrah J. Kassetas, Friederike Baumgaertner, James D. Kirsch, Sheri T. Dorsam, Tammi L. Neville, J. Chris Forcherio, Ronald R. Scott, Joel S. Caton, Carl R. Dahlen
Department of Animal Science: Faculty Publications
Maternal nutrients are essential for proper fetal and placental development and function. However, the effects of vitamin and mineral supplementation under two rates of maternal weight gain on placental genome-wide gene expression have not been investigated so far. Furthermore, biological processes and pathways in the placenta that act in response to early maternal nutrition are yet to be elucidated. Herein, we examined the impact of maternal vitamin and mineral supplementation (from pre-breeding to day 83 post-breeding) and two rates of gain during the first 83 days of pregnancy on the gene expression of placental caruncles (CAR; maternal placenta) and cotyledons …
Gene Expression Profiling Of Bovine Ovarian Follicular And Luteal Cells Provides Insight Into Cellular Identities And Functions, Sarah Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A. Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp
Gene Expression Profiling Of Bovine Ovarian Follicular And Luteal Cells Provides Insight Into Cellular Identities And Functions, Sarah Romereim, Adam F. Summers, William E. Pohlmeier, Pan Zhang, Xiaoying Hou, Heather A. Talbott, Robert A. Cushman, Jennifer R. Wood, John S. Davis, Andrea S. Cupp
Department of Animal Science: Faculty Publications
After ovulation, somatic cells of the ovarian follicle (theca and granulosa cells) become the small and large luteal cells of the corpus luteum. Aside from known cell type-specific receptors and steroidogenic enzymes, little is known about the differences in the gene expression profiles of these four cell types. Analysis of the RNA present in each bovine cell type using Affymetrix microarrays yielded new cell-specific genetic markers, functional insight into the behavior of each cell type via Gene Ontology Annotations and Ingenuity Pathway Analysis, and evidence of small and large luteal cell lineages using Principle Component Analysis. Enriched expression of select …