Meat Science Commons^™

Organic Production Of Livestock And Livestock Products In Nebraska, Elliott James Dennis

Extension Farm and Ranch Management News

First paragraph:

USDA “Certified Organic” is a form of production that has received considerable public attention in the last 10 years, particularly among small, beginning and/or minority farmers. Spiking retail prices for red meat and poultry have seemingly accelerated consumer’s interest in organic locally produced products. Consumers demonstrate their desire for these products by paying premiums when purchasing meat products. Producers receive premiums by either directly marketing live animals to consumers that are custom harvested or marketing animals to meat wholesalers. The primary difference is the way premiums are captured. Producers doing custom harvesting capture the full premium whereas producers …

A Transgenic Pig Model Expressing A Cmv-Zsgreen1 Reporter Across An Extensive Array Of Tissues, Amy T. Desaulniers, Rebecca A. Cederberg, Elizabeth P. Carreiro, Channabasavaiah B. Gurumurthy, Brett R. White

Department of Animal Science: Faculty Publications

Since genetic engineering of pigs can benefit both biomedicine and agriculture, selecting a suitable gene promoter is critically important. The cytomegalovirus (CMV) promoter, which can robustly drive ubiquitous transgene expression, is commonly used at present, yet recent reports suggest tissue-specific activity in the pig. The objective of this study was to quantify ZsGreen1 protein (in lieu of CMV promoter activity) in tissues from pigs harboring a CMV-ZsGreen1 transgene with a single integration site. Tissue samples (n=35) were collected from neonatal hemizygous (n=3) and homozygous (n=3) piglets and ZsGreen1 abundance was determined via immunoblotting. ZsGreen1 was detected in all tissues, except …

Using Triallelic Snps For Determining Parentage In North American Yak (Bos Grunniens) And Estimating Cattle (B. Taurus) Introgression, Theodore S. Kalbfleisch, Jessica L. Petersen, R. G. Tait Jr., Jiansheng Qiu, Veronica Basnayake, Peter H. Hackett, Michael P. Heaton

Department of Animal Science: Faculty Publications

Background: Genetic testing for pedigree accuracy is critical for managing genetic diversity in North American (NA) yak (Bos grunniens ), a population expanded mostly from imported zoological park specimens. DNA testing also enhances species conservation by identifying recent B. taurus F1 hybrid ancestors (within three generations). Biallelic single nucleotide polymorphisms (SNPs) can accomplish either task, but increases the marker count and costs necessary to achieve both. Our aim was to identify novel, multifunctional, triallelic yak SNPs (tySNPs), with each having two alleles for yak parentage testing, and a third allele for identifying recent cattle introgression.

Methods: Genome sequences were …

Mandibulofacial Dysostosis Attributed To A Recessive Mutation Of Cyp26c1 In Hereford Cattle, Renae L. Sieck, Anna M. Fuller, Patrick S. Bedwell, Jack A. Ward, Stacy K. Sanders, Shi-Hua Xiang, Sichong Peng, Jessica Lynn Petersen, David J. Steffen

Department of Animal Science: Faculty Publications

In spring 2020, six Hereford calves presented with congenital facial deformities attributed to a condition we termed mandibulofacial dysostosis (MD). Affected calves shared hallmark features of a variably shortened and/or asymmetric lower mandible and bilateral skin tags present 2–10 cm caudal to the commissure of the lips. Pedigree analysis revealed a single common ancestor shared by the sire and dam of each affected calf. Whole-genome sequencing (WGS) of 20 animals led to the discovery of a variant (Chr26 g. 14404993T>C) in Exon 3 of CYP26C1 associated with MD. This missense mutation (p.L188P), is located in an α helix of …

Impact Of Mixed Biofilm Formation With Environmental Microorganisms On E. Coli O157:H7 Survival Against Sanitization, Sapna Chitlapilly Dass, Joseph M. Bosilevac, Maggie Weinroth, Christian Elowsky, You Zhou, Angela Anandappa, Rong Wang

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Biofilm formation by foodborne pathogens is a serious threat to food safety and public health. Meat processing plants may harbor various microorganisms and occasional foodborne pathogens; thus, the environmental microbial community might impact pathogen survival via mixed biofilm formation. We collected floor drain samples from two beef plants with different E. coli O157:H7 prevalence history and investigated the effects of the environmental microorganisms on pathogen sanitizer tolerance. The results showed that biofilm forming ability and bacterial species composition varied considerably based on the plants and drain locations. E. coli O157:H7 cells obtained significantly higher sanitizer tolerance in mixed biofilms by …

Effect Of Method Of Drying Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A.S. Alencar, Michael Ellis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Piglets are born wet, and evaporation of that moisture decreases body temperature, increasing the risk of mortality. The objective of this study was to compare the effect of two commercially applicable methods for drying piglets at birth on piglet rectal temperature over 24 h after birth. The study was carried out in standard commercial farrowing facilities with 52 litters, using a completely randomized design with three Drying Treatments: Control (not dried); Desiccant (dried at birth using a cellulose-based desiccant); Paper Towel (dried at birth using paper towels). Litters were randomly allotted to treatments at the birth of the first piglet. …

Effect Of Drying And/Or Warming Piglets At Birth On Rectal Temperature Over The First 24 H After Birth, Katherine D. Vande Pol, Andres F. Tolosa, Caleb M. Shull, Catherine B. Brown, Stephan A. S. Alencar, Michael Ellis

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Piglets experience a rapid decrease in body temperature immediately after birth, increasing the risk of mortality. The objective of this study was to determine the effect of drying and/or warming piglets at birth on rectal temperature over the first 24 h after birth. The study was carried out at a commercial sow facility using a completely randomized design with four treatments (applied to piglets at birth): Control (no drying or warming), Desiccant (dried using a desiccant), Warming Box (placed in a box under a heat lamp for 30 min), and Desiccant + Warming Box (both dried and warmed as above). …

Genomic Analysis Of Igg Antibody Response To Common Pathogens In Commercial Sows In Health-Challenged Herds, Leticia P. Sanglard, Piggen Canada, Benny E. Mote, Philip Willson, John C.S. Harding, Graham S. Plastow, Jack C.M. Dekkers, Nick V.L. Serão

Department of Animal Science: Faculty Publications

Losses due to infectious diseases are one of the main factors affecting productivity in the swine industry, motivating the investigation of disease resilience-related traits for genetic selection. However, these traits are not expected to be expressed in the nucleus herds, where selection is performed. One alternative is to use information from the commercial level to identify and select nucleus animals genetically superior for coping with pathogen challenges. In this study, we analyzed the genetic basis of antibody (Ab) response to common infectious pathogens in health-challenged commercial swine herds as potential indicator traits for disease resilience, including Ab response to influenza …

Uncertainties Abound In Nebraska Livestock Market Forecasts, Elliott James Dennis

Extension Farm and Ranch Management News

About one of the only things certain about forecasts this year has been the great deal of uncertainty. Numerous “novel” situations have confronted the industry making it difficult to estimate price magnitudes rather than just price direction. Clustered plant closures, government quarantine restrictions, and increasing at home phone consumption are just a few of the major issues the beef industry has had to tackle in the last six to eight months. Some things have returned to “normal” such as packing plant capacity and food service demand. Others are still in ongoing recovery.

Who knows what “normal” or “standard operating procedure” …

Comparing Profitability And Management Factors Across Operation Type: Independent Vs. Contract Growing, Elliott James Dennis

Extension Farm and Ranch Management News

First paragraph:

There is strong local and national interest in adding hog barns to existing row crop operations. Declining on farm income over the past several years has accelerated this interest. Grain operations cite diversifying farm income, adding another family to the farm operation, and/or replacing commercial fertilizer expense as the primary reasons for adding a hog barn.

A Historical Perspective On The Holcomb Fire: Differences And Similarities To The Covid-19 Situation And Other Significant Market Events, Elliott James Dennis

Extension Farm and Ranch Management News

Copyright 2020, the author. Used by permission.

Hog Barn Density And Location In U.S. And Nebraska, Elliott James Dennis

Extension Farm and Ranch Management News

There is strong local and national interest in adding hog barns to existing row crop operations. Declining on farm income over the past several years has accelerated this interest. Grain operations cite diversifying farm income, adding another family to the farm operation, and/or replace commercial fertilizer expense as the primary reasons for adding a hog barn.

While there is industry demand to increase hog contract growing, these agreements can possess significant benefits and risks to producers. This is the first of a four part series which will discuss some financial and legal implications of adding a hog barn to an …

Free Calcium Concentration, Calpain-2 Activity, And Final Product Tenderness Of Electrically Stimulated Beef, B J. Buseman, T M. Weber, J A. Nasados, P D. Bass, J B. Van Buren, J M. Lancaster, J H. Smart, M E. Doumit, G K. Murdoch, W J. Price, K Insausti, M J. Colle

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the effect of timing of electrical stimulation on free calcium concentration, calpain-2 activity, Warner-Bratzler shear force (WBSF), and consumer sensory analysis. Twenty-three beef steers were harvested and stimulated (S) using extra-low voltage or not stimulated (NS), at exsanguination and/or 1 h postmortem, resulting in 4 different stimulation treatments: NS-NS, NS-S, S-NS, or S-S. Samples were removed from the longissimus lumborum (LL) and semimembranosus (SM) for free calcium and calpain-2 analysis on days 1, 4, and 14 postmortem. WBSF and sensory analysis steaks were removed on day 4 and frozen (4 d) or …

Assessing Outcomes Of Genetic Selection Panels To Predict Marbling In Crossbred Beef Cattle, Tanya M. Weber, B J. Buseman, James A. Nasados, Jessica M. Lancaster, Jessie B. Van Buren, Jaxon H. Smart, Phillip D. Bass, Gordon K. Murdoch, Kizkitza Insausti, Michael J. Colle

Department of Animal Science: Faculty Publications

The objective of this study was to evaluate the effectiveness of genetic panel marbling indexes [Igenity (IT) and PredicGEN (PG)] to predict marbling and tenderness of crossbred cattle. Steers (n = 23) were harvested at the University of Idaho Meat Science Laboratory, and blood samples were submitted to Neogen and Zoetis for genetic panel analysis. Forty-eight hours postharvest, one boneless strip loin was collected from each carcass, and six 2.54-cm thick steaks were cut from each strip loin. Steaks were aged for 14 and 21 d and assigned to consumer sensory evaluation or Warner–Bratzler Shear Force (WBSF) analysis. Results were …

Air Temperature, Carbon Dioxide, And Ammonia Assessment Inside A Commercial Cage Layer Barn With Manure-Drying Tunnels, W Zheng, Y Xiong, R S. Gates, K W. Koelkebeck

Department of Animal Science: Faculty Publications

Understanding the air temperature distribution, ammonia (NH₃) and carbon dioxide (CO₂) levels in poultry housing systems are crucial to poultry health, welfare, and productivity. In this study, 4 Intelligent Portable Monitoring Units and 7 temperature sensors were installed inside and between the cages and above 2 minimum ventilation fans of a commercial stacked-deck cage laying hen house in the Midwest United States (425,000 laying hens) to continuously monitor the interior environment over a 6-month period. During cold conditions (March 12th–May 22nd), there was a variation noted, with barn center temperatures consistently being highest in the longitudinal …

Using Genetic Panels To Predict Tenderness In Beef Cattle, Tanya M. Weber, Michael J. Colle, Gordon K. Murdoch, B J. Buseman, Jessica M. Lancaster, Jessie B. Van Buren, James A. Nasados, Phillip D. Bass

Department of Animal Science: Faculty Publications

Genetic panel use as a selection tool has grown in popularity in the beef industry. The objective of the study was to determine whether beef cattle genetically selected for tenderness generated a tender product. Igenity® (IT) panel results were provided by a cattle producer for 52 steers, which were harvested at a commercial harvest facility. Boneless strip loins (Institutional Meat Purchase Specifications #180; United States Department of Agriculture [USDA] Choice, n = 32; USDA Prime n = 20) were collected from the left side of each carcass and transported to the University of Idaho Meat Science Laboratory. Four steaks were …

The Impact Of Oxidative Stress On Postmortem Meat Quality, Nicolas J. Herrera

Department of Animal Science: Dissertations, Theses, and Student Research

Tenderness is the primary factor to influence consumer palatability in fresh meat. The tenderization of muscle is dependent on proteolytic mechanisms, including calcium dependent, non-lysosomal endogenous enzymes such as calpains and proteasomes. These mechanisms have been indicated alongside apoptosis early postmortem as cascades of apoptotic events promote ideal conditions for postmortem tenderization. Recent literature has identified oxidative stress-mediated events to be linked to apoptotic activity. Oxidative stress is defined as the overwhelming production of reactive species compared to homeostatic, endogenous antioxidants present within muscle systems. These conditions exhaust antioxidant function and facilitate reactive species to alter protein, lipid, or nucleic …

Effects Of A Vitamin And Mineral Bolus On Beef Heifer Feedlot Performance, Feeding Behavior, Carcass Characteristics, And Liver Mineral Concentrations, Kacie L. Mccarthy, Sarah R. Underdahl, Carl R. Dahlen

Department of Animal Science: Faculty Publications

Crossbred beef heifers (n = 23; initial body weight [BW] = 370 ± 12 kg) housed at the North Dakota State University Beef Cattle Research Complex in Fargo, North Dakota, were used to evaluate the effects of a 250-d slow-release vitamin and mineral bolus on feedlot performance, feeding behavior, carcass characteristics, and liver mineral concentrations. Heifers were assigned to one of two treatments: 1) received no supplemental mineral or vitamin (CON, n = 12 or 2) received two boluses on day 0 (minimum of 3,740 mg Ca, 16,456 mg Mg, 112 mg Na, 11,220 mg Cu, 2,995 mg I, …

Pigs And Poultry, Nicole Burton

Sustainability Education Resources

This course provides students with a farmer’s perspective on the management, production and marketing of poultry and pigs on a small farm. This course will address the advantages of having pigs and poultry and will review basic care, processing options, regulations, marketing and finances. The course will be structured around lectures, farm visits, guest lectures and acquiring hands on skills. We will explore different alternative farming systems that utilize animals to enhance their production. At the end of this course, students will be able to utilize pigs and poultry as an integral part of their small farm plan.

Small Farm Husbandry: Cow, Sheep And Goat For Meat Production, Nicole Burton

Sustainability Education Resources

This course provides students with a farmer’s perspective on the sustainable management of cows, sheep and goats on a small farm. The course provides students with a clear understanding of how to think through the planning and management of cows, sheep and goats for meat production. All aspects from purchasing and nutrition to marketing and finances will be addressed. This course also brings awareness to the U.S. meat industry and supports students in becoming educated consumers and producers. Students will walk away with a rudimentary plan on how to incorporate ruminants into their small farm plan.

The “Challenge" Of Depletion: Why The Oyster Fishery Is Not Self-Regulating, Eric N. Powell, John M. Klinck, Leanne M. Poussard

CCPO Publications

The possibility that the economics of the oyster fishery impose a self-limitation on overharvesting has been proffered on occasion. The inefficiency of harvesting by the fishery has been evaluated and estimates of the exploitation rate permissible under conditions of maximum sustainable yield have been obtained in previous studies. The question becomes to what extent does the inefficiency of harvest interact with the economics of the fishery to compromise ready detection of overfishing? This study explores the possibility that the constraint of economics on the fishery occurs at oyster exploitation rates that are higher than maximum sustainable yield, leading ineluctably to …

Comparison Of Poly-A+ Selection And Rrna Depletion In Detection Of Lncrna In Two Equine Tissues Using Rna-Seq, Anna R. Dahlgren, Erica Y. Scott, Tamer Mansour, Erin N. Hales, Pablo J. Ross, Theodore S. Kalbfleisch, James N. Macleod, Jessica Lynn Petersen, Rebecca R. Bellone, Carrie J. Finno

Department of Animal Science: Faculty Publications

Long non-coding RNAs (lncRNAs) are untranslated regulatory transcripts longer than 200 nucleotides that can play a role in transcriptional, post-translational, and epigenetic regulation. Traditionally, RNA-sequencing (RNA-seq) libraries have been created by isolating transcriptomic RNA via poly-A+ selection. In the past 10 years, methods to perform ribosomal RNA (rRNA) depletion of total RNA have been developed as an alternative, aiming for better coverage of whole transcriptomic RNA, both polyadenylated and non-polyadenylated transcripts. The purpose of this study was to determine which library preparation method is optimal for lncRNA investigations in the horse. Using liver and cerebral parietal lobe tissues from two …

Effect Of Ammoniation And Harvest Method On Waste And Consumption Of Corn Residue Bales Fed To Cows In A Round Bale Feeder, Ashley C. Conway, Zachary E. Carlson, Fred H. Hilscher Jr, J. C. Macdonald, Terry J. Klopfenstein, Mary E. Drewnoski

Department of Animal Science: Faculty Publications

To determine the effects of harvest method and ammoniation (3.7% of dry matter) on consumption and waste of baled corn residue, a 6 × 6 Latin square with a 3 × 2 factorial treatment structure was conducted. Six treatments consisted of either nonammoniated or ammoniated residue, harvested one of three ways: conventional rake and bale (CONV), New Holland Cornrower with two rows of stem chopped into the windrow with tailings (2ROW), or EZBale system (EZB) with a disengaged combine spreader and tailings dropped in a windrow. Open cows were grouped by body weight to produce a light block of two …

Asas Celebrates 10 Years Of Publishing Animal Frontiers: The Review Magazine Of Animal Agriculture, Debora L. Hamernik, Meghan Wulster-Radcliffe, Steven A. Zinn

Department of Animal Science: Faculty Publications

In July 2011, the first issue of Animal Frontiers was published by an international consortium including the American Society of Animal Science (ASAS), the Canadian Society of Animal Science (CSAS), and the European Federation of Animal Science (EAAP). Development of an international, science-based, open access journal that provided review articles and global perspectives on the complex dynamics associated with the production of animal-sourced foods was an accomplishment in and of itself, but the ability to meet the needs of a diverse community of readers, including scientists, policy makers, educators, and the general public set Animal Frontiers apart from other journals. …

The Effects Of 2 Genetic Lines On Spatial Distribution And Use And Preference Of Perch And Nest Area In An Aviary System, Sheila E. Scheideler, Pamela E. Eusebio Balcazar, K. Hanford

Department of Animal Science: Faculty Publications

The objective of this trial was to evaluate spatial distribution, nest and perch usage, and preference of Lohmann Brown (BH) and Bovan White (WH) Leghorn hens raised in an aviary system. At 5 wk, 400 floor raised pullets, BH and WH strains, in equal numbers, were placed into 8 modified Big Dutchman Natura aviary units. Each aviary unit had 3 tiers with perches and an indoor litter area. At 25 wk, the number of birds was recorded by scan sampling every 4 h. The number of birds perching in each aviary unit was recorded every 4 h at 15, 25, …

Impact Of Cow Milk Production On Cow–Calf Performance In The Nebraska Sandhills, Tasha M. King, Jacki A. Musgrave, Richard N. Funston, J. Travis Mulliniks

Department of Animal Science: Faculty Publications

Livestock producers have tended to select for increased output traits like milk production and growth to increase productivity. Even with the increased selection for greater calf growth potential, some regions in the United States have seen a plateau in calf body weight (BW) at weaning (Lalman et al., 2019). When focusing on reaching maximum potential of these output traits, it is important to consider the multitude of variables that affect a production system. With increased milk production, nutrient requirements for cows become increased (Ferrell and Jenkins, 1984; Montaño-Bermudez et al., 1990), which may not be met if range and forage …

Formation And Characterization Of Lipid Droplets Of The Bovine Corpus Luteum, Heather A. Talbott, Michele R. Plewes, Crystal Krause, Xiaoying Hou, Pan Zhang, William B. Rizzo, Jennifer R. Wood, Andrea S. Cupp, John S. Davis

Department of Animal Science: Faculty Publications

Establishment and maintenance of pregnancy depends on progesterone synthesized by luteal tissue in the ovary. Our objective was to identify the characteristics of lipid droplets (LDs) in ovarian steroidogenic cells. We hypothesized that LDs are a major feature of steroidogenic luteal cells and store cholesteryl esters. Whole bovine tissues, isolated ovarian steroidogenic cells (granulosa, theca, small luteal, and large luteal), and isolated luteal LDs were assessed for LD content, LD-associated proteins and lipid analyses. Bovine luteal tissue contained abundant lipid droplets, LD-associated perilipins 2/3/5, hormone-sensitive lipase, and 1-acylglycerol-3-phosphate O-acyltransferase ABHD5. Luteal tissue was enriched in triglycerides (TGs) compared to other …

Heat Stress And Β-Adrenergic Agonists Alter The Adipose Transcriptome And Fatty Acid Mobilization In Ruminant Livestock, Rachel R. Reith, Renae L. Sieck, Pablo C. Grijalva, Erin M. Duffy, Rebecca M. Swanson, Anna M. Fuller, Kristin A. Beede, Joslyn K. Beard, Duarte E. Diaz, Ty B. Schmidt, Dustin T. Yates, Jessica L. Petersen

Department of Animal Science: Faculty Publications

Growth and feed efficiency of cattle are improved by supplementation with the beta-adrenergic agonists (βAA), ractopamine hydrochloride (RH; β₁AA) or zilpaterol hydrochloride (ZH; β₂AA) (Elam et al., 2009). βAA supplementation alters adipose deposition by inhibiting fatty acid biosynthesis and promoting lipolysis of stored triacylglycerols into free fatty acids (FFAs) (Johnson et al., 2014). However, β₂ adrenoceptors (βAR) desensitize with chronic activation (Re et al., 1997); supplementation is thus limited to the last 20 to 40 d of feeding.

The annual economic impact of heat stress (HS) has been estimated to exceed $2.4 billion (St-Pierre et …

Beef Cows With Atypical Estrous Cyclicity At Puberty Produced Calves With Deficits In Preweaning Muscling, Metabolic Indicators, And Myoblast Function But Not In Feedlot Performance, Rebecca M. Swanson, Rachel L. Gibbs, Caitlin Cadaret, Galen E. Erickson, Ty B. Schmidt, Andrea Cupp, Dustin T. Yates

Department of Animal Science: Faculty Publications

In cattle, age at puberty and number of estrous cycles prior to first breeding contribute to lifetime reproductive success (Perry et al., 1991). In our university beef herd, we have identified a subset of cows that exhibited irregular pubertal cyclicity patterns between weaning and their first breeding season, which we postulate is associated with high androstenedione in follicular fluid (Cupp et al., 2019). Cows with high androstenedione are subfertile but wean calves that average 17 kg heavier than the herd average (Summers et al., 2014). We hypothesized that this additional weight at weaning in their offspring is due to superior …

Influence Of Host Genetics In Shaping The Rumen Bacterial Community In Beef Cattle, Waseem Abbas, Jeremy T. Howard, Henry Paz, Kristin E. Hales, James E. Wells, Larry A. Kuehn, Galen E. Erickson, Matthew L. Spangler, Samodha Fernando

Department of Animal Science: Faculty Publications

In light of recent host-microbial association studies, a consensus is evolving that species composition of the gastrointestinal microbiota is a polygenic trait governed by interactions between host genetic factors and the environment. Here, we investigated the effect of host genetic factors in shaping the bacterial species composition in the rumen by performing a genome-wide association study. Using a common set of 61,974 single-nucleotide polymorphisms found in cattle genomes (n = 586) and corresponding rumen bacterial community composition, we identified operational taxonomic units (OTUs), Families and Phyla with high heritability. The top associations (1-Mb windows) were located on 7 chromosomes. These …