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Full-Text Articles in Meat Science

Cell Surface Charge And Initial Attachment Characteristics Of Rough Strains Of Listeria Monocytogenes, James S. Dickson Jan 1994

Cell Surface Charge And Initial Attachment Characteristics Of Rough Strains Of Listeria Monocytogenes, James S. Dickson

James S. Dickson

The relative negative surface charge and hydrophobicity of four bacterial strains were evaluated by gravity flow and spin column methods. There was no significant difference between the two methods, indicating that spin column chromatography is an acceptable alternative method of determining cell surface charge or hydrophobicity. Six strains of Listeria monocytogenes which exhibited rough colony appearance were evaluated for surface charge and hydrophobicity and their ability to contaminate beef muscle tissue. With one exception, all of the rough strains exhibited greater net negative surface charge and reduced ability to contaminate beef during the initial stages of attachment. Since greater net …


Antimicrobial Effects Of Trisodium Phosphate Against Bacteria Attached To Beef Tissue, James S. Dickson, C. G. Nettles Cutter, G. R. Siragusa Jan 1994

Antimicrobial Effects Of Trisodium Phosphate Against Bacteria Attached To Beef Tissue, James S. Dickson, C. G. Nettles Cutter, G. R. Siragusa

James S. Dickson

Sliced beef tissue was artificially contaminated with Salmonella typhimurium, Listeria monocytogenes and Escherichia coli OI57:H7. The contaminated tissue was immersed in 8, 10 and 12% solutions of trisodium phosphate at 25, 40 and 55°C with contact times of up to 3 min. The concentration of the trisodium phosphate solution was not a significant factor in reducing the populations of the bacteria on either lean or adipose tissue. Reductions in bacterial populations of I to 1.5 loglocycles were obtained on lean tissue contaminated with the gram-negative pathogens, although less reduction in population was seen with L. monocytogenes. Greater reductions in bacterial …