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Full-Text Articles in Dairy Science
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
Masters Theses
In vitro produced (IVP) bovine embryos provide enhanced genetic value and a means for studying early embryonic development. However, IVP embryos are developmentally compromised and are lower quality compared to in vivo derived (IVD) embryos. Consequently, after transfer pregnancy rates from IVP embryos are consistently lower, as they exhibit the highest amounts of pregnancy failure during the early embryonic phase (prior to day 28). Progesterone (P4) has been shown to influence bovine endometrial epithelial (EPI) and stromal fibroblast (SF) cells to secrete developmentally important molecules that promote early embryo development and conceptus elongation. However, IVP embryos are not exposed to …
Impact Of Matrix Metallopeptidase-9 Supplementation During In Vitro Maturation Of Bovine Oocytes, Megan Rene Goodwin
Impact Of Matrix Metallopeptidase-9 Supplementation During In Vitro Maturation Of Bovine Oocytes, Megan Rene Goodwin
Masters Theses
Heat-induced decreases in bovine blastocyst development have been related to reductions in latent matrix metallopeptidase-9 (proMMP9) production from maturing cumulus-oocyte complexes. Elevated intrafollicular proMMP9 levels at the time of oocyte retrieval have been positively related to pregnancy following human IVF. Thus, we hypothesized that heat-induced reductions in proMMP9 levels during oocyte maturation may be responsible for decreased blastocyst development. As a first step towards testing this hypothesis, bovine cumulus-oocyte complexes were matured at 38.5°C for 24 h with 0 or 300 ng/mL recombinant human proMMP9 (rhMMP9) added at 0 h of in vitro maturation (hIVM). No differences were found in …