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Full-Text Articles in Dairy Science
Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey
Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Embryos resulting from assisted reproductive technologies, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), develop with lower efficiencies than embryos resulting from their in vivo counterparts. The reasons behind the developmental discrepancies remain largely unknown. Because the egg is the primary determinant of embryo developmental success, it is reasonable to consider inherent egg quality as a possible cause. The hypothesis for this project is that there are distinct mRNA transcript patterns, or molecular “fingerprints,” that distinguish high- versus low-quality eggs developing within the same environment. In this study, a small cytoplasmic biopsy was removed from 40 eggs …
Impact Of Ph And Palmitic Acid On Ruminal Fermentation And Microbial Community Composition, Lexie Padilla
Impact Of Ph And Palmitic Acid On Ruminal Fermentation And Microbial Community Composition, Lexie Padilla
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The objective of this study was to evaluate the effects of dietary palmitic acid and pH on rumen fermentation, fiber digestibility, and bacterial community composition. The two factors in the experiment were palmitic acid treatment and pH treatment. Palmitic acid treatments included a control diet compared to a diet containing 1.5% palmitic acid. pH treatments included normal pH (6.6 to 7.0) compared to low pH (6.0 to 6.4). Rumen fluid from a cow was added to artificial rumens to study the effects of the two treatments relative to fermentation and changes within the microbial community. Results of the study showed …