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Full-Text Articles in Dairy Science
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
The Effect Of Bovine Endometrial Cell Conditioned Media On In Vitro Produced Bovine Embryo Development And Gene Expression, Mary A. Oliver
Masters Theses
In vitro produced (IVP) bovine embryos provide enhanced genetic value and a means for studying early embryonic development. However, IVP embryos are developmentally compromised and are lower quality compared to in vivo derived (IVD) embryos. Consequently, after transfer pregnancy rates from IVP embryos are consistently lower, as they exhibit the highest amounts of pregnancy failure during the early embryonic phase (prior to day 28). Progesterone (P4) has been shown to influence bovine endometrial epithelial (EPI) and stromal fibroblast (SF) cells to secrete developmentally important molecules that promote early embryo development and conceptus elongation. However, IVP embryos are not exposed to …
Assessing Mitochondrial Activity In Embryos From Heat-Stressed Ova, Kimberly Ann Nagle
Assessing Mitochondrial Activity In Embryos From Heat-Stressed Ova, Kimberly Ann Nagle
Masters Theses
Elevated ambient temperatures negatively impacts pregnancy rates in agriculturally important females. An oocyte undergoing meiotic maturation exposed to a physiologically-relevant heat stress results in alterations of developmentally important processes and reportedly decreases subsequent developmental potential after fertilization. To address problems of reduced competence after exposure to heat stress for the first 12 h of meiotic maturation (hIVM), Payton (2009) assessed the transcriptome profiles of oocytes and associated cumulus cells by microarray analysis and reported heat-induced perturbations at the molecular level in the oocyte. Specifically, 20 transcripts involved in mitochondrial function exhibited alterations in relative abundance of heat-stressed oocytes …