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Validation Of Antibodies Used To Study Hypoxia Inducible Factors In Two Species Of Fundulus, Jenna D. Hill May 2013

Validation Of Antibodies Used To Study Hypoxia Inducible Factors In Two Species Of Fundulus, Jenna D. Hill

University of New Orleans Theses and Dissertations

Hypoxia inducible factors (HIFs) are transcription factors and the master regulators of oxygen-dependent gene expression in animals. The focus of this thesis is the distribution of HIF protein in tissues of the fish Fundulus heteroclitus and F. grandis, two widespread species that occur in naturally hypoxic waters. Polyclonal antibodies against HIF-1α, HIF-2α, and HIF-3α were tested on proteins made in vitro and on extracts made from several tissues of normoxic and hypoxic fish. Antibodies against HIF-1α and 3α bound specifically to full length protein made in vitro, and produced bands on western blots of nuclear extracts of near …


Ecology Of Glacial Relict Fishes In South Dakota's Sandhills Region, Eli Felts Jan 2013

Ecology Of Glacial Relict Fishes In South Dakota's Sandhills Region, Eli Felts

Electronic Theses and Dissertations

Native stream fish zoogeography has changed substantially across North America during the last century as habitat degradation, stream fragmentation and introductions of nonnative species have led to numerous extinctions, extirpations and altered distributions. Insufficient information regarding imperiled species often results in reactive, rather than proactive, management, and knowledge of species status and ecology is critical in identifying conservation priorities. South Dakota populations of three dace species (northern redbelly dace Chrosomus eos, finescale dace Chrosomus neogaeus, and pearl dace Margariscus margarita) are relict of Pleistocene Glaciation and are isolated from the northern core of their distribution, but little information exists regarding …